Abstract

The goal of this proposal is to study the dynamics of Ca++ trafficking through cellular organelles and its relationship to the regulation of exocytosis using a combination of high time resolution patch clamp, capacitance measurements, amperometry, photometry and imaging. This will be done in single cells and adrenal slice preparations.
The specific aims are: 1. Dynamics of Ca++ movement into and out of organelles. Specific hypotheses addressed are that organelles play significant roles in Ca++ homeostasis and that chromaffin secretory granules are Ca++ releasing and sequestering organelles. 2. Signaling pathway for regulated exocytosis in epithelia. The hypothesis that a cAMP-dependent pathway controls exocytosis will be tested. 3. Physiology of chromaffin cells. The hypothesis that chromaffin secretion is regulated by paracrine and modulatory actions will be tested.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Research Project (R01)
Project #
5R01AR017803-26
Application #
6171679
Study Section
Physiology Study Section (PHY)
Program Officer
Lymn, Richard W
Project Start
1977-09-01
Project End
2002-03-31
Budget Start
2000-04-01
Budget End
2001-03-31
Support Year
26
Fiscal Year
2000
Total Cost
$231,693
Indirect Cost

  Institution

Name
University of Washington
Department
Physiology
Type
Schools of Medicine
DUNS #
135646524
City
Seattle
State
WA
Country
United States
Zip Code
98195

  Publications

Kim, Mean-Hwan; Seo, Jong Bae; Burnett, Lindsey A et al. (2013) Characterization of store-operated Ca2+ channels in pancreatic duct epithelia. Cell Calcium 54:266-75
Cho, Jung-Hwa; Chen, Liangyi; Kim, Mean-Hwan et al. (2010) Characteristics and functions of {alpha}-amino-3-hydroxy-5-methyl-4-isoxazolepropionate receptors expressed in mouse pancreatic {alpha}-cells. Endocrinology 151:1541-50
Duman, Joseph G; Chen, Liangyi; Hille, Bertil (2008) Calcium transport mechanisms of PC12 cells. J Gen Physiol 131:307-23
Lauckner, Jane E; Jensen, Jill B; Chen, Huei-Ying et al. (2008) GPR55 is a cannabinoid receptor that increases intracellular calcium and inhibits M current. Proc Natl Acad Sci U S A 105:2699-704
Suh, Byung-Chang; Hille, Bertil (2007) Regulation of KCNQ channels by manipulation of phosphoinositides. J Physiol 582:911-6
Suh, Byung-Chang; Inoue, Takanari; Meyer, Tobias et al. (2006) Rapid chemically induced changes of PtdIns(4,5)P2 gate KCNQ ion channels. Science 314:1454-7
Duman, Joseph G; Chen, Liangyi; Palmer, Amy E et al. (2006) Contributions of intracellular compartments to calcium dynamics: implicating an acidic store. Traffic 7:859-72
Koh, Duk-Su (2006) Carbon fiber amperometry in the study of ion channels and secretion. Methods Mol Biol 337:139-53
Jung, Seung-Ryoung; Kim, Kyungjin; Hille, Bertil et al. (2006) Pattern of Ca2+ increase determines the type of secretory mechanism activated in dog pancreatic duct epithelial cells. J Physiol 576:163-78
Horowitz, Lisa F; Hirdes, Wiebke; Suh, Byung-Chang et al. (2005) Phospholipase C in living cells: activation, inhibition, Ca2+ requirement, and regulation of M current. J Gen Physiol 126:243-62

Showing the most recent 10 out of 53 publications