Abstract

1. CYTOGENETIC AND BIOLOGICAL STUDIES I.1 Plasmacytomagenesis a) Characterization of the MPC-precursor cell. b) Continued studies on the MPC-accelerating role of A-MuLV. c) The genetics of MPC susceptibility at the target cell level. I.2 Tumor suppression a) Murine system: Analysis of individual chr mediated suppressor effects. b) Human system: Suppression of clonability and tumorigenicity in human BL cells. II. MOLECULAR STUDIES II.1 Functional studies on myc a) Continued studies on the effect of c-myc on SV40 replication: i) Definition of c-myc domains involved in the stimulation of SV40 replication; ii) Identification of protein(s) that interact with c-myc in this system. b) SV40-Rb-myc interactions: i) Can c-myc interfere with the negative effect of Rb on SV40 replication? ii) Is the growth suppression by the Rb gene product abrogated by constitutive c-myc expression? II.2 Continued studies on a myc associated protein kinase activity. II.3 Continued studies on the Ig/myc translocations: Molecular analysis of the new 15;16 and the 6;12 translocations in MPC; Analysis of myc transcription in RICs and MPCs with distant breakpoints. II.4 Continued studies on B-myc: Effect on the SV40 system; differentiation-related studies. III. AUXILIARY STUDIES: Are Rb or p53 changed in Ig-myc translocation-carrying tumors?

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA014054-21
Application #
2086204
Study Section
Mammalian Genetics Study Section (MGN)
Project Start
1980-01-01
Project End
1995-07-31
Budget Start
1994-08-01
Budget End
1995-07-31
Support Year
21
Fiscal Year
1994
Total Cost
Indirect Cost

  Institution

Name
Karolinska Institute
Department
Type
DUNS #
350582235
City
Stockholm
State
Country
Sweden
Zip Code
171 7-7

  Publications

Silva, S; Wang, Y; Babonits, M et al. (1997) Spontaneous development of plasmacytomas in a selected subline of BALB/cJ mice. Eur J Cancer 33:479-85
Szekely, L; Pokrovskaja, K; Klein, G (1997) Differential expression of nucleoskeleton- and cytoskeleton-associated proteins in Burkitt lymphoma-derived and Epstein-Barr virus-immortalized lymphoblastoid cell lines. Cell Growth Differ 8:599-609
Sumegi, J; Wang, J Y; Zhen, D K et al. (1996) The construction of a yeast artificial chromosome (YAC) contig in the vicinity of the Usher syndrome type IIa (USH2A) gene in 1q41. Genomics 35:79-86
Allikmets, R; Kashuba, V I; Huebner, K et al. (1996) Mapping of 22 Notl linking clones on human chromosome 3 by polymerase chain reaction and somatic cell hybrid panels. Chromosome Res 4:33-7
Sithanandam, G; Latif, F; Duh, F M et al. (1996) 3pK, a new mitogen-activated protein kinase-activated protein kinase located in the small cell lung cancer tumor suppressor gene region. Mol Cell Biol 16:868-76
Szekely, L; Pokrovskaja, K; Jiang, W Q et al. (1996) The Epstein-Barr virus-encoded nuclear antigen EBNA-5 accumulates in PML-containing bodies. J Virol 70:2562-8
Szeles, A; Bajalica-Lagercrantz, S; Lindblom, A et al. (1996) Mapping of a new MAP kinase activated protein kinase gene (3PK) to human chromosome band 3p21.2 and ordering of 3PK and two cosmid markers in the 3p22-p21 tumour-suppressor region by two-colour fluorescence in situ hybridization. Chromosome Res 4:310-3
Vorobieva, N; Protopopov, A; Protopopova, M et al. (1995) Localization of human ARF2 and NCK genes and 13 other NotI-linking clones to chromosome 3 by fluorescence in situ hybridization. Cytogenet Cell Genet 68:91-4
Axelson, H; Henriksson, M; Wang, Y et al. (1995) The amino-terminal phosphorylation sites of C-MYC are frequently mutated in Burkitt's lymphoma lines but not in mouse plasmacytomas and rat immunocytomas. Eur J Cancer 31A:2099-104
Kashuba, V I; Szeles, A; Allikmets, R et al. (1995) A group of NotI jumping and linking clones cover 2.5 Mb in the 3p21-p22 region suspected to contain a tumor suppressor gene. Cancer Genet Cytogenet 81:144-50

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