Abstract

In order to gain an understanding of the nature of hormonal dependency and autonomy of breast cancers, a basic understanding of the action of hormones at the cellular and subcellulr level will be required. Two experimental mammary tumors in rodents are being studied. To examine the actions of insulin at the cellular level, we will employ dissociated tumor cells from hormonally modified animals, and will examine in vitro the characteristics of insulin binding to its receptor, the actions of insulin on glucose and amino acid transport, the utilization of the transported substrates and the effects of estrogen and other hormones on the regulation of insulin binding and its action in these tumors. Short-term tissue culture of tumor cells, which has been initiated successfully, will be used to examine hormonal regulaton of insulin and estrogen binding in vitro, and the effects of these hormones on growth and metabolism of these cells. Since we perceive that insulin has both direct and permissive actions, the ultimate goal of the studies is to define these actions of insulin and determine how these may be modified by other hormones, and to relate such mechanisms to tumor growth and its control.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA016660-09
Application #
3164449
Study Section
Biochemical Endocrinology Study Section (BCE)
Project Start
1978-09-30
Project End
1988-03-03
Budget Start
1985-04-01
Budget End
1986-03-31
Support Year
9
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of Rochester
Department
Type
Schools of Medicine
DUNS #
208469486
City
Rochester
State
NY
Country
United States
Zip Code
14627

  Publications

Jung, Sung Keun; Kim, Jong Eun; Lee, Sung-Young et al. (2014) The P110 subunit of PI3-K is a therapeutic target of acacetin in skin cancer. Carcinogenesis 35:123-30
Liu, Haidan; Hwang, Joonsung; Li, Wei et al. (2014) A derivative of chrysin suppresses two-stage skin carcinogenesis by inhibiting mitogen- and stress-activated kinase 1. Cancer Prev Res (Phila) 7:74-85
Liu, Haidan; Liu, Kangdong; Huang, Zunnan et al. (2013) A chrysin derivative suppresses skin cancer growth by inhibiting cyclin-dependent kinases. J Biol Chem 288:25924-37
Song, Nu Ry; Lee, Eunjung; Byun, Sanguine et al. (2013) Isoangustone A, a novel licorice compound, inhibits cell proliferation by targeting PI3K, MKK4, and MKK7 in human melanoma. Cancer Prev Res (Phila) 6:1293-303
Li, Yufeng; Matsueda, Satoko; Efferson, Clayton L et al. (2009) Distinct patient responses to activation of T-cells by free HER-2, G89 (777-789) and protected LRMK-linked HER-2, {AE-39 [p776 (Ava-774-788)], AE-47 [(Ava-776-788)] and AE-37[p776 (774-788)]} peptides could lead to development of personalized cancer vacci Anticancer Res 29:41-58
Matsueda, Satoko; Gao, Hui; Efferson, Clayton L et al. (2009) N-terminally LRMK-linked HER-2 peptides, AE-37 [p776(774-788)] and AE-47 [Ava-F7(776-788)], aid differentiation of E75-TCR+CD8+ cells to perforin-positive cells. Anticancer Res 29:2427-35
Li, Yufeng; Ishiyama, Satoshi; Matsueda, Satoko et al. (2008) HER-2 peptides p776 and F7, N-terminal-linked with Ii-Key tetramer (LRMK) help the proliferation of E75-TCR+ cells: The dependency of help on the side chains of LRMK-extended peptide pointed towards the T cell receptor. Oncol Rep 19:1445-52
Sathya, G; Li, W; Klinge, C M et al. (1997) Effects of multiple estrogen responsive elements, their spacing, and location on estrogen response of reporter genes. Mol Endocrinol 11:1994-2003
Korc-Grodzicki, B; Ren, N; Hilf, R (1996) Effects of estradiol on the expression and production of IGFBP-2 by R3230AC mammary tumor cells. Oncol Res 8:473-83
Korc-Grodzicki, B; Ren, N; Hilf, R (1993) Insulin-like growth factor-binding proteins in R3230AC mammary tumors of intact and diabetic rats. Endocrinology 133:2362-70

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