Abstract

The goals of this proposal are to continue trying to understand in molecular detail how retroviruses interact with their hosts to cause diseases, in particular, cancer. An intrinsic hope of this line of work is that this understanding will ultimately contribute to the prevention or treatment of viral induced diseases. The specific goals of the proposal are 1) To continue our detailed genetic and biochemical analysis of the enhancers of mouse retroviruses in order to understand how these elements interact with cellular proteins to encode biological properties of the viruses including the type of leukemia they induce. 2) To apply a similar analysis to the LTR of the simian AIDS virus SIV in order to learn whether this element also plays an important role in disease induction by the virus. 3) To continue trying to isolate genes that encode retrovirus receptors, particularly the MCF virus receptor, in order both to learn about virus-receptor interactions and to probe the role of MCF viruses in mouse retrovirus induced leukemias.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA019308-15
Application #
3165133
Study Section
Virology Study Section (VR)
Project Start
1976-06-30
Project End
1993-11-30
Budget Start
1990-12-01
Budget End
1991-11-30
Support Year
15
Fiscal Year
1991
Total Cost
Indirect Cost

  Institution

Name
Massachusetts Institute of Technology
Department
Type
Organized Research Units
DUNS #
City
Cambridge
State
MA
Country
United States
Zip Code
02139

  Publications

Kawakami, K; Hopkins, N (1996) Rapid identification of transgenic zebrafish. Trends Genet 12:9-10
Gaiano, N; Allende, M; Amsterdam, A et al. (1996) Highly efficient germ-line transmission of proviral insertions in zebrafish. Proc Natl Acad Sci U S A 93:7777-82
Amsterdam, A; Lin, S; Moss, L G et al. (1996) Requirements for green fluorescent protein detection in transgenic zebrafish embryos. Gene 173:99-103
Amsterdam, A; Lin, S; Hopkins, N (1995) The Aequorea victoria green fluorescent protein can be used as a reporter in live zebrafish embryos. Dev Biol 171:123-9
Manley, N R; O'Connell, M; Sun, W et al. (1993) Two factors that bind to highly conserved sequences in mammalian type C retroviral enhancers. J Virol 67:1967-75
Winandy, S; Renjifo, B; Li, Y et al. (1992) Nuclear factors that bind two regions important to transcriptional activity of the simian immunodeficiency virus long terminal repeat. J Virol 66:5216-23
Hunter, K; Housman, D; Hopkins, N (1991) Isolation and characterization of irradiation fusion hybrids from mouse chromosome 1 for mapping Rmc-1, a gene encoding a cellular receptor for MCF class murine retroviruses. Somat Cell Mol Genet 17:169-83
Golemis, E A; Speck, N A; Hopkins, N (1990) Alignment of U3 region sequences of mammalian type C viruses: identification of highly conserved motifs and implications for enhancer design. J Virol 64:534-42
Renjifo, B; Speck, N A; Winandy, S et al. (1990) cis-acting elements in the U3 region of a simian immunodeficiency virus. J Virol 64:3130-4
Speck, N A; Renjifo, B; Golemis, E et al. (1990) Mutation of the core or adjacent LVb elements of the Moloney murine leukemia virus enhancer alters disease specificity. Genes Dev 4:233-42

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