The early proteins of SV-40 large T antigen and small t antigen are model viral oncogenes and have been studied from both biochemical and genetic approaches. We propose to search for cellular components which may interact with these viral products through the continuation of a broad based program. Specifically, we propose to: 1. Obtain cell lines which have reverted from oncogenic transformation. These lines may habor mutation in genes required for transformation. The appropriate revertants may be used as tools for cloning these putative cellular genes via transfection protocols. 2. We would like to know if certain cellular sequences which bind viral T antigen are linked to genes which may be induced by SV-40, and more generally, we have designed transient expression assays to ask if T binding to DNA is a positive activator or heterologous promoters. 3. Clone c-DNA copies of rare mRNA species that are expressed at elevated levels in SV-40 transformed cells. 4. Study the nature of cell cycle regulated expression of the cellular Tk gene, as a model gene which is indirectly or directly induced by acute viral infection.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA030490-07
Application #
3169272
Study Section
Experimental Virology Study Section (EVR)
Project Start
1981-07-01
Project End
1987-12-31
Budget Start
1987-01-01
Budget End
1987-12-31
Support Year
7
Fiscal Year
1987
Total Cost
Indirect Cost
Name
University of California Berkeley
Department
Type
Schools of Arts and Sciences
DUNS #
094878337
City
Berkeley
State
CA
Country
United States
Zip Code
94704
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