Abstract

description). This 11A2 application will employ the ML-1 human myeloblastic leukemia cell line to study the molecular responses to specific growth versus differentiation cytokines this laboratory has identified in this model. These include phospholipid pathways and lipid-derived second messengers formed from them; the role of the c-ets-1 protein in regulating c-myb expression; the type and level of cell cycle regulating proteins such as cyclins, cyclin-dependent kinases and tumor repressors. In addition experiments are proposed to examine the effect of these cytokines on differentiation in bone marrow from patients with AML in the presence of specific antitumor agents.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA036241-13
Application #
2608014
Study Section
Experimental Therapeutics Subcommittee 1 (ET)
Program Officer
Forry-Schaudies, Suzanne L
Project Start
1984-06-01
Project End
1999-11-30
Budget Start
1997-12-01
Budget End
1999-11-30
Support Year
13
Fiscal Year
1998
Total Cost
Indirect Cost

  Institution

Name
Roswell Park Cancer Institute Corp
Department
Type
DUNS #
City
Buffalo
State
NY
Country
United States
Zip Code
14263

  Publications

Mudipalli, A; Li, Z; Hromchak, R et al. (2001) NF-kappaB (p65/RelA) as a regulator of TNFalpha-mediated ML-1 cell differentiation. Leukemia 15:808-13
Li, Z R; Hromchak, R; Mudipalli, A et al. (1998) Tumor suppressor proteins as regulators of cell differentiation. Cancer Res 58:4282-7
Li, Z; Hromchak, R; Bloch, A (1997) Differential expression of proteins regulating cell cycle progression in growth vs. differentiation. Biochim Biophys Acta 1356:149-59
Bloch, A; Liu, X M; Wang, L G (1995) Regulation of c-myb expression in ML-1 human myeloblastic leukemia cells by c-ets-1 protein. Adv Enzyme Regul 35:35-41
Wang, L G; Liu, X M; Li, Z R et al. (1994) Differential binding of nuclear c-ets-1 protein to an intron I fragment of the c-myb gene in growth versus differentiation. Cell Growth Differ 5:1243-51
Wikiel, H; Zhao, L; Gessner, T et al. (1994) Differential effect of growth- and differentiation-inducing factors on the release of eicosanoids and phospholipids from ML-1 human myeloblastic leukemia cells. Biochim Biophys Acta 1211:161-70
Bloch, A (1993) Dynamics of interaction between DNA-specific antitumor agents and serum-contained cytokines in the initiation of ML-1 human myeloblastic leukemia cell differentiation. Leukemia 7:1219-24
Denstman, S; Hromchak, R; Guan, X P et al. (1991) Identification of transferrin as a progression factor for ML-1 human myeloblastic leukemia cell differentiation. J Biol Chem 266:14873-6
Guan, X P; Hromchak, R A; Takuma, T et al. (1991) Tumor necrosis factor-alpha, transforming growth factor-beta, and tetradecanoylphorbol acetate: competence factors for ML-1 human myeloblastic leukemia cell differentiation. Cancer Commun 3:11-4
Fujii, Y; Takuma, T; Bloch, A (1990) A regulatory role for tumor necrosis factor (TNF) in ML-1 human myeloblastic leukemia cell maturation. Leuk Res 14:941-7

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