Abstract

The objective of this research is to provide an understanding of the structural and functional features of the polyoma virus origin region and those gene products which enable the virus to replicate its DNA, transcribe RNA's and transform cells in culture. Existing deletion and insertion mutants, together with newly isolated point mutants will be used to characterize the regulation of RNA transcription, splicing and DNA replication. These processes will be correlated with the physical structure of this region (organized into nucleosomes). T-antigens of polyoma virus will be purified from bacterial and/or animal cells containing modified viral genomes which permit high efficiency expression. The capacity of these purified proteins to interact with the modified viral origin region will be explored.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA038538-03
Application #
3176590
Study Section
Virology Study Section (VR)
Project Start
1984-09-01
Project End
1987-06-30
Budget Start
1986-01-01
Budget End
1987-06-30
Support Year
3
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
University of Texas Austin
Department
Type
Schools of Arts and Sciences
DUNS #
City
Austin
State
TX
Country
United States
Zip Code
78713

  Publications

Xie, An-Yong; Folk, William R (2002) Inhibition of polyomavirus ori-dependent DNA replication by mSin3B. J Virol 76:11809-18
Xie, An-Yong; Bermudez, Vladimir P; Folk, William R (2002) Stimulation of DNA replication from the polyomavirus origin by PCAF and GCN5 acetyltransferases: acetylation of large T antigen. Mol Cell Biol 22:7907-18
Berjanskii, M V; Riley, M I; Xie, A et al. (2000) NMR structure of the N-terminal J domain of murine polyomavirus T antigens. Implications for DnaJ-like domains and for mutations of T antigens. J Biol Chem 275:36094-103
Riley, M I; Yoo, W; Mda, N Y et al. (1997) Tiny T antigen: an autonomous polyomavirus T antigen amino-terminal domain. J Virol 71:6068-74
Guo, W; Tang, W J; Bu, X et al. (1996) AP1 enhances polyomavirus DNA replication by promoting T-antigen-mediated unwinding of DNA. J Virol 70:4914-8
Li, L; Li, B L; Hock, M et al. (1995) Sequences flanking the pentanucleotide T-antigen binding sites in the polyomavirus core origin help determine selectivity of DNA replication. J Virol 69:7570-8
Lednicky, J; Folk, W R (1992) Two synthetic Sp1-binding sites functionally substitute for the 21-base-pair repeat region to activate simian virus 40 growth in CV-1 cells. J Virol 66:6379-90
Kang, S; Folk, W R (1992) Lymphotropic papovavirus transforms hamster cells without altering the amount or stability of p53. Virology 191:754-64
Scanlon, S R; Folk, W R (1991) Nuclease Bal-31 mapping of proteins bound to a tRNA(tyr) gene in SV40 minichromosomes. Nucleic Acids Res 19:7185-92
Tang, W J; Folk, W R (1989) Asp-286----Asn-286 in polyomavirus large T antigen relaxes the specificity of binding to the polyomavirus origin. J Virol 63:242-9

Showing the most recent 10 out of 16 publications