Abstract

Otitis media with effusion (OME) is one of the largest public health problems of young children. The inflammatory events in OME lead to effusion and tissue hyperplasia, producing temporary or permanent hearing loss. Immune responses play a major role in OME, since immunity is intimately involved in the host response to infection of the middle ear (ME). Inflammatory events based upon response to antigen have also been implicated in OME pathogenesis. Issues to be studied in the present application include whether immune response to bacterial antigens prolongs OME after antibiotic treatment. We will also investigate whether antibiotic treatment of bacterial OME decreases the sensitization of immunity by reducing the level and duration of bacterial antigens present in the ME. The cellular and molecular mechanisms which control immune responses in the ME will be studied, with a goal of determining whether ME immunity and resistance to infection can be increased, and immune-mediated inflammation can be decreased. Finally, we will study the contributions of growth factors in generating ME tissue hyperplasia during OME. We will identify substances that promote tissue proliferation and differentiation, and which are present in the ME during OME. We will also investigate the response of the ME mucosa to direct application of growth factors.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute on Deafness and Other Communication Disorders (NIDCD)
Type
Research Project (R01)
Project #
5R01DC000129-13
Application #
3215794
Study Section
Hearing Research Study Section (HAR)
Project Start
1990-04-01
Project End
1995-03-31
Budget Start
1991-04-01
Budget End
1992-03-31
Support Year
13
Fiscal Year
1991
Total Cost
Indirect Cost

  Institution

Name
University of California San Diego
Department
Type
Schools of Medicine
DUNS #
077758407
City
La Jolla
State
CA
Country
United States
Zip Code
92093

  Publications

Kurabi, Arwa; Schaerer, Daniel; Chang, Lisa et al. (2018) Optimisation of peptides that actively cross the tympanic membrane by random amino acid extension: a phage display study. J Drug Target 26:127-134
Kurabi, Arwa; Schaerer, Daniel; Noack, Volker et al. (2018) Active Transport of Peptides Across the Intact Human Tympanic Membrane. Sci Rep 8:11815
Kurabi, Arwa; Beasley, Kerry A; Chang, Lisa et al. (2017) Peptides actively transported across the tympanic membrane: Functional and structural properties. PLoS One 12:e0172158
Deniffel, Dominik; Nuyen, Brian; Pak, Kwang et al. (2017) Otitis Media and Nasopharyngeal Colonization in ccl3-/- Mice. Infect Immun 85:
Cho, Chang Gun; Pak, Kwang; Webster, Nicholas et al. (2016) Both canonical and non-canonical NF-?B activation contribute to the proliferative response of the middle ear mucosa during bacterial infection. Innate Immun 22:626-634
Kurabi, Arwa; Pak, Kwang K; Bernhardt, Marlen et al. (2016) Discovery of a Biological Mechanism of Active Transport through the Tympanic Membrane to the Middle Ear. Sci Rep 6:22663
Hernandez, Michelle; Leichtle, Anke; Pak, Kwang et al. (2015) The transcriptome of a complete episode of acute otitis media. BMC Genomics 16:259
Leichtle, Anke; Klenke, Christin; Ebmeyer, Joerg et al. (2015) NOD-Like Receptor Signaling in Cholesteatoma. Biomed Res Int 2015:408169
Kurabi, Arwa; Lee, Jasmine; Wong, Chelsea et al. (2015) The inflammasome adaptor ASC contributes to multiple innate immune processes in the resolution of otitis media. Innate Immun 21:203-14
Yao, William; Frie, Meredith; Pan, Jeffrey et al. (2014) C-Jun N-terminal kinase (JNK) isoforms play differing roles in otitis media. BMC Immunol 15:46

Showing the most recent 10 out of 25 publications