Secretory Immune Response &S. Mutans Glucosyltransferas

Taubman, Martin A.

Abstract

An aim of this research is to examine the effects of enhances secretory antibody directed to glucosyltransferase (GTF) on plaque and dental caries. Studies in hamsters will determine the effect of local immunization with S. mutans GFT on infection with S. sanguis. Enzume linked imuunosorbent assay (ELISA) will be used for secretory antibody determinations to GTF. Studies of the secretory IgA response to GTF will also involve modes of enhancing the response with adjuvants. Various orally active adjuvants will be tested in combination with GTF. Cellular aspects of secretory IgA response will be tested with GTF as antigen. A plaque forming cell assay for GTF will be developed. Cell transfers and antibody-forming cells in organs will be monitored with an ELISA technique with cells in culture. Studies of the role of the T cell in the secretory immune response will be pursued in in vivo reconstitution experiments with thymus cells. Also the effects of enhanced secretory immunity (adjuvants) and the effects of T cell deprivation on dental caries will be studied. The long term goals of this proposal are to increase understanding of the secretory immune response so that this reponse could most effectively be utilized to combat dental infections. Also it is particularly important to optimize the effectiveness of GTF as a vaccine administered orally or by injection. Immunological and microbiological methodology will be employed in order to facilitate the development of a vaccine effective against dental caries.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Dental & Craniofacial Research (NIDCR)
• Type: Research Project (R01)
• Project #: 5R01DE004733-08
• Application #: 3219130
• Study Section: Oral Biology and Medicine Study Section (OBM)
• Project Start: 1977-07-01
• Project End: 1986-11-30
• Budget Start: 1985-04-01
• Budget End: 1986-11-30
• Support Year: 8
• Fiscal Year: 1985

Institution

• Name: Forsyth Institute
• City: Cambridge
• State: MA
• Country: United States

Publications

Han, Xiaozhe; LaRosa, Karen B; Kawai, Toshihisa et al. (2014) DNA-based adaptive immunity protect host from infection-associated periodontal bone resorption via recognition of Porphyromonas gingivalis virulence component. Vaccine 32:297-303

Smith, Daniel J (2010) Dental caries vaccines: prospects and concerns. Expert Rev Vaccines 9:1-3

Nogueira, R D; King, W F; Gunda, G et al. (2008) Mutans streptococcal infection induces salivary antibody to virulence proteins and associated functional domains. Infect Immun 76:3606-13

Culshaw, S; Larosa, K; Tolani, H et al. (2007) Immunogenic and protective potential of mutans streptococcal glucosyltransferase peptide constructs selected by major histocompatibility complex class II allele binding. Infect Immun 75:915-23

Smith, Daniel J; King, William F; Rivero, Joy et al. (2005) Immunological and protective effects of diepitopic subunit dental caries vaccines. Infect Immun 73:2797-804

Russell, Michael W; Childers, Noel K; Michalek, Suzanne M et al. (2004) A Caries Vaccine? The state of the science of immunization against dental caries. Caries Res 38:230-5

Smith, D J; Lam, A; Barnes, L A et al. (2003) Remote glucosyltransferase-microparticle vaccine delivery induces protective immunity in the oral cavity. Oral Microbiol Immunol 18:240-8

Smith, Daniel J (2003) Caries vaccines for the twenty-first century. J Dent Educ 67:1130-9

Smith, D J (2002) Dental caries vaccines: prospects and concerns. Crit Rev Oral Biol Med 13:335-49

Eastcott, J W; Holmberg, C J; Dewhirst, F E et al. (2001) Oligonucleotide containing CpG motifs enhances immune response to mucosally or systemically administered tetanus toxoid. Vaccine 19:1636-42