Understanding the structure of bone is the foundation upon which advances can be made in determining the processes involved in its formation, and the factors responsible for its malformation. It is proposed to substantiate and evaluate our recently discovered structural model of bone, in which the crystals in individual lamellae are primarily arranged in parallel layers. Of particular interest, will be the comparisons between the structures of bones performing different function sin the same animal (the rat), and between Haversian and non-Haversian bone, as well as the determination of the stages of crystal growth in association wit collagen fibrils (mineralizing turkey tendon). Both electron diffraction and imaging in the transmission electron microscope (TEM) will be extensively used, as well as scanning electron microscopy. The transition will then be made to probing processes of crystal formation in bone, using a novel """"""""all-cryo"""""""" methodology for immunochemical mapping of various non-collagenous acidic macromolecular constituents in relation to crystal formation. A key question will be the presence of such components at the nucleation site. Cryo-sectioned mineralized tissues will be studied unstained and """"""""hydrated"""""""" in the vitrified state using the TEM. The structural information from normal bones will be compared with that from bones suffering pathology, in order to identify any significant differences, and hopefully relate these to the manner in which the tissue malfunctions.

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Research Project (R01)
Project #
5R01DE006954-08
Application #
3220457
Study Section
Oral Biology and Medicine Subcommittee 1 (OBM)
Project Start
1985-03-01
Project End
1994-05-31
Budget Start
1992-08-20
Budget End
1993-05-31
Support Year
8
Fiscal Year
1992
Total Cost
Indirect Cost
Name
Weizmann Institute of Science
Department
Type
DUNS #
City
Rehovot
State
Country
Israel
Zip Code
76100
Utku, F Sermin; Klein, Eugenia; Saybasili, Hale et al. (2008) Probing the role of water in lamellar bone by dehydration in the environmental scanning electron microscope. J Struct Biol 162:361-7
Weiner, Stephen (2008) Biomineralization: a structural perspective. J Struct Biol 163:229-34
Shahar, R; Zaslansky, P; Barak, M et al. (2007) Anisotropic Poisson's ratio and compression modulus of cortical bone determined by speckle interferometry. J Biomech 40:252-64
Zaslansky, Paul; Friesem, Asher A; Weiner, Steve (2006) Structure and mechanical properties of the soft zone separating bulk dentin and enamel in crowns of human teeth: insight into tooth function. J Struct Biol 153:188-99
Zaslansky, Paul; Currey, John D; Friesem, Asher A et al. (2005) Phase shifting speckle interferometry for determination of strain and Young's modulus of mineralized biological materials: a study of tooth dentin compression in water. J Biomed Opt 10:024020
Wood, Judy D; Wang, Rizhi; Weiner, Steve et al. (2003) Mapping of tooth deformation caused by moisture change using moire interferometry. Dent Mater 19:159-66
Addadi, L; Weiner, S; Geva, M (2001) On how proteins interact with crystals and their effect on crystal formation. Z Kardiol 90 Suppl 3:92-8
Beniash, E; Traub, W; Veis, A et al. (2000) A transmission electron microscope study using vitrified ice sections of predentin: structural changes in the dentin collagenous matrix prior to mineralization. J Struct Biol 132:212-25
Liu, D; Weiner, S; Wagner, H D (1999) Anisotropic mechanical properties of lamellar bone using miniature cantilever bending specimens. J Biomech 32:647-54
Weiner, S; Traub, W; Wagner, H D (1999) Lamellar bone: structure-function relations. J Struct Biol 126:241-55

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