Pituitary Tumor Cell Cultures

Melmed, Shlomo

Abstract

This proposal is aimed at studying the long-term culture of human and rat functional pituitary tumor cells and somatic cell hybrids secreting growth hormone or prolactin. Hormone modulation by thyrotropin-releasing hormone (TRH), growth hormone releasing factor (GRF), thyroid hormones and other neurotransmitters will be studied. The human pituitary cell cultures will provide an in vitro model for studying mechanisms of hormone synthesis and release and provide further insights into the regulation of human prolactin and growth hormone secretion, the pathophysiology of pituitary tumors and female and male reproductive control and infertility.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
• Type: Research Project (R01)
• Project #: 5R01DK033802-03
• Application #: 3232202
• Study Section: Endocrinology Study Section (END)
• Project Start: 1984-09-30
• Project End: 1987-08-31
• Budget Start: 1986-09-01
• Budget End: 1987-08-31
• Support Year: 3
• Fiscal Year: 1986

Institution

• Name: Cedars-Sinai Medical Center
• DUNS #: 075307785
• City: Los Angeles
• State: CA
• Country: United States
• Zip Code: 90048

Publications

Bousquet, C; Melmed, S (1999) Critical role for STAT3 in murine pituitary adrenocorticotropin hormone leukemia inhibitory factor signaling. J Biol Chem 274:10723-30

Yamasaki, H; Prager, D; Gebremedhin, S et al. (1992) Human insulin-like growth factor I receptor 950tyrosine is required for somatotroph growth factor signal transduction. J Biol Chem 267:20953-8

Melmed, S; Ziel, F H; Braunstein, G D et al. (1988) Medical management of acromegaly due to ectopic production of growth hormone-releasing hormone by a carcinoid tumor. J Clin Endocrinol Metab 67:395-9

Fagin, J A; Brown, A; Melmed, S (1988) Regulation of pituitary insulin-like growth factor-I messenger ribonucleic acid levels in rats harboring somatomammotropic tumors: implications for growth hormone autoregulation. Endocrinology 122:2204-10

Morita, S; Yamashita, S; Melmed, S (1987) Insulin-like growth factor I action on rat anterior pituitary cells: effects of intracellular messengers on growth hormone secretion and messenger ribonucleic acid levels. Endocrinology 121:2000-6

Yamashita, S; Ong, J; Melmed, S (1987) Regulation of human growth hormone gene expression by insulin-like growth factor I in transfected cells. J Biol Chem 262:13254-7