Abstract

The long term objective is to assess the biological activity, metabolism and teratogenicity of some naturally-occurring and synthetic water-soluble derivatives of vitamin A.
Specific aims are: 1) to synthesize radioactivity labeled retinoyl beta-glucuronide, retinyl beta-glucuronide, retinyl beta-glucose and several other water-soluble analogs, 2) to ascertain how these water-soluble analogs are metabolized, 3) to determine their biological activity and physiologically active forms in growth and in cellular differentiation, and 4) to measure their teratogenicity in golden hamsters and their cytoxicity towards selected cell lines in tissue culture. The planned studies have direct relevance to vitamin A utilization in disorders of lipid absorption, e.g., cholestasis, to the treatment of skin disorders and possibly to the chemoprevention of recurrent neoplasms.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
5R01DK039733-02
Application #
3239649
Study Section
Metabolic Pathology Study Section (MEP)
Project Start
1988-03-01
Project End
1991-02-28
Budget Start
1989-03-01
Budget End
1990-02-28
Support Year
2
Fiscal Year
1989
Total Cost
Indirect Cost

  Institution

Name
Iowa State University
Department
Type
Schools of Arts and Sciences
DUNS #
City
Ames
State
IA
Country
United States
Zip Code
50011

  Publications

Barua, Arun B; Sidell, Neil (2004) Retinoyl beta-glucuronide: a biologically active interesting retinoid. J Nutr 134:286S-289S
Barua, Arun B (2003) Absorption and conversion of a single oral dose of beta-carotene in corn oil to vitamin A in Sprague-Dawley rats with low reserve of vitamin A. Int J Vitam Nutr Res 73:267-73
Sidell, N; Pasquali, M; Malkapuram, S et al. (2003) In vitro and in vivo effects of easily administered, low-toxic retinoid and phenylacetate compounds on human neuroblastoma cells. Br J Cancer 89:412-9
Goswami, Bhabesh C; Ivanoff, Kristi D; Barua, Arun B (2003) Absorption and conversion of 11,12-(3)H-beta-carotene to vitamin A in Sprague-Dawley rats of different vitamin A status. J Nutr 133:148-53
Barua, Arun B (2003) A simple rapid method of sample preparation for LC analysis of retinoyl beta-glucuronide and retinoic acid in water-based creams. J Pharm Biomed Anal 32:563-7
Goswami, Bhabesh C; Reida, Abigail K; Ivanoff, Kristi D et al. (2003) Intestinal absorption and metabolism of retinoyl beta-glucuronide in humans, and of 15-[14C]-retinoyl beta-glucuronide in rats of different vitamin A status. J Nutr Biochem 14:703-9
Romans, David A; Barua, Arun B; Olson, James Allen (2003) Pharmacokinetics of all-trans retinoyl beta-glucuronide in rats following intraperitoneal and oral administration. Int J Vitam Nutr Res 73:251-7
Ueltschy, Amanda; Gunning, Desiree B; Barua, Arun B et al. (2002) Effects of subcutaneously injected graded doses of all-trans retinoic acid and all-trans retinoyl beta-glucuronide on the outcome of pregnancy in Sprague-Dawley rats. Int J Vitam Nutr Res 72:229-35
Gunning, Desiree B; Barua, Arun B; Myers, Ronald K et al. (2002) Comparative histologic effects of daily topical application of creams containing all-trans-retinoic acid or all-trans-retinoyl beta-glucuronide on pig skin. Skin Pharmacol Appl Skin Physiol 15:205-12
Barua, A B (2001) Improved normal-phase and reversed-phase gradient high-performance liquid chromatography procedures for the analysis of retinoids and carotenoids in human serum, plant and animal tissues. J Chromatogr A 936:71-82

Showing the most recent 10 out of 52 publications