Abstract

The long-term goal of this application is the understanding of the mechanisms of thyroid hormone (T3) action with focus on the roles of T3 receptors (TR) in the regulation of gene expression.
The specific aims of this application are to: (1) identify TR amino acids required specifically for both the formation of heterodimers between TRs and the retinoid X receptors (RXRs), and for transcriptional activation by such heterodimeric complexes; (2) identify new proteins that interact specifically with TRs in the presence and absence of T3, and characterize their roles in TR function; and (3) define the in vivo occupancy of T3 and other response elements by TRs and other members of the nuclear hormone receptor superfamily.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
2R01DK043382-04
Application #
2142974
Study Section
Reproductive Endocrinology Study Section (REN)
Project Start
1991-08-01
Project End
1998-07-31
Budget Start
1994-08-01
Budget End
1995-07-31
Support Year
4
Fiscal Year
1994
Total Cost
Indirect Cost

  Institution

Name
Massachusetts General Hospital
Department
Type
DUNS #
City
Boston
State
MA
Country
United States
Zip Code
02199

  Publications

Pissios, P; Tzameli, I; Kushner, P et al. (2000) Dynamic stabilization of nuclear receptor ligand binding domains by hormone or corepressor binding. Mol Cell 6:245-53
Koh, Y S; Moore, D D (1999) Linkage of the nuclear hormone receptor genes NR1D2, THRB, and RARB: evidence for an ancient, large-scale duplication. Genomics 57:289-92
Na, S Y; Kim, H J; Lee, S K et al. (1998) IkappaBbeta interacts with the retinoid X receptor and inhibits retinoid-dependent transactivation in lipopolysaccharide-treated cells. J Biol Chem 273:3212-5
Seol, W; Chung, M; Moore, D D (1997) Novel receptor interaction and repression domains in the orphan receptor SHP. Mol Cell Biol 17:7126-31
Martinez, E; Moore, D D; Keller, E et al. (1997) The Nuclear Receptor Resource Project. Nucleic Acids Res 25:163-5
Zuo, F; Kraus, R J; Gulick, T et al. (1997) Direct modulation of simian virus 40 late gene expression by thyroid hormone and its receptor. J Virol 71:427-36
Seol, W; Mahon, M J; Lee, Y K et al. (1996) Two receptor interacting domains in the nuclear hormone receptor corepressor RIP13/N-CoR. Mol Endocrinol 10:1646-55
Lee, J W; Choi, H S; Gyuris, J et al. (1995) Two classes of proteins dependent on either the presence or absence of thyroid hormone for interaction with the thyroid hormone receptor. Mol Endocrinol 9:243-54
Seol, W; Choi, H S; Moore, D D (1995) Isolation of proteins that interact specifically with the retinoid X receptor: two novel orphan receptors. Mol Endocrinol 9:72-85
Baes, M; Gulick, T; Choi, H S et al. (1994) A new orphan member of the nuclear hormone receptor superfamily that interacts with a subset of retinoic acid response elements. Mol Cell Biol 14:1544-52

Showing the most recent 10 out of 13 publications