The goal of this proposal is to understand interactions between intestinal epithelial cells and neutrophils that occurs during intestinal inflammation. An approach has been used with in vitro epithelial monolayers and peripheral blood neutrophils. The specific new aims are to determine the compartment in neutrophils which release 5' AMP and study the biology of the epithelial ectoenzyme CD73 and receptor A2b for adenosine. Second, studies of the role of CD47 in epithelial cell neutrophil interactions will be studied by overexpressing CD47 in epithelial cells and expressing dominant negative mutants. A novel epithelial ligand will be defined, and finally the role of intraepithelial lymphocytes will be defined in a mixed cell culture system.
The third aim will be to identify novel factors expressed during bacterial infection of epithelial cells, and finally the ability of long acting analogs of lipoxins to inhibit neutrophil interactions and murine colitis will be studied.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
5R01DK047662-07
Application #
2770444
Study Section
General Medicine A Subcommittee 2 (GMA)
Program Officer
Hamilton, Frank A
Project Start
1993-09-30
Project End
2002-08-31
Budget Start
1998-09-01
Budget End
1999-08-31
Support Year
7
Fiscal Year
1998
Total Cost
Indirect Cost
Name
Emory University
Department
Pathology
Type
Schools of Medicine
DUNS #
042250712
City
Atlanta
State
GA
Country
United States
Zip Code
30322
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