Abstract

The long-term goal of this project is to further the understanding of the role of the transcription factor C/EBPalpha in regulating adipocyte gene expression and metabolism. C/EBPalpha is induced during the differentiation of 3T3-L preadipocytes into adipocytes and activates the expression of genes involved in creating and maintaining the adipocyte phenotype, notably the insulin responsive glucose transporter Glut-4, adipocyte lipid binding protein 422/AP2, and stearoyl CoA desaturase 1 (SCD1) genes. The PI will test the effects of insulin-induced changes in phosphorylation on the activity of C/EBPalpha and elucidate the role of C/EBPalpha in regulating the expression of downstream adipocyte genes.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
5R01DK051563-03
Application #
2734222
Study Section
Endocrinology Study Section (END)
Program Officer
Haft, Carol R
Project Start
1996-07-25
Project End
2000-06-30
Budget Start
1998-07-25
Budget End
1999-06-30
Support Year
3
Fiscal Year
1998
Total Cost
Indirect Cost

  Institution

Name
University of Michigan Ann Arbor
Department
Physiology
Type
Schools of Medicine
DUNS #
791277940
City
Ann Arbor
State
MI
Country
United States
Zip Code
48109

  Publications

Cawthorn, William P; Bree, Adam J; Yao, Yao et al. (2012) Wnt6, Wnt10a and Wnt10b inhibit adipogenesis and stimulate osteoblastogenesis through a ?-catenin-dependent mechanism. Bone 50:477-89
Mori, Hiroyuki; Prestwich, Tyler C; Reid, Michael A et al. (2012) Secreted frizzled-related protein 5 suppresses adipocyte mitochondrial metabolism through WNT inhibition. J Clin Invest 122:2405-16
Akhmetshina, Alfiya; Palumbo, Katrin; Dees, Clara et al. (2012) Activation of canonical Wnt signalling is required for TGF-?-mediated fibrosis. Nat Commun 3:735
Cawthorn, William P; Scheller, Erica L; MacDougald, Ormond A (2012) Adipose tissue stem cells meet preadipocyte commitment: going back to the future. J Lipid Res 53:227-46
Cawthorn, William P; Scheller, Erica L; MacDougald, Ormond A (2012) Adipose tissue stem cells: the great WAT hope. Trends Endocrinol Metab 23:270-7
Bommer, Guido T; MacDougald, Ormond A (2011) Regulation of lipid homeostasis by the bifunctional SREBF2-miR33a locus. Cell Metab 13:241-7
Susperreguy, Sebastián; Prendes, Luciana P; Desbats, María A et al. (2011) Visualization by BiFC of different C/EBP? dimers and their interaction with HP1? reveals a differential subnuclear distribution of complexes in living cells. Exp Cell Res 317:706-23
Wei, Jun; Melichian, Denisa; Komura, Kazuhiro et al. (2011) Canonical Wnt signaling induces skin fibrosis and subcutaneous lipoatrophy: a novel mouse model for scleroderma? Arthritis Rheum 63:1707-17
Wen, Xin; Cawthorn, William P; MacDougald, Ormond A et al. (2011) The influence of Leucine-rich amelogenin peptide on MSC fate by inducing Wnt10b expression. Biomaterials 32:6478-86
Clark, Anna M; Sousa, Kyle M; Chisolm, Claire N et al. (2010) Reversibly sealed multilayer microfluidic device for integrated cell perfusion and on-line chemical analysis of cultured adipocyte secretions. Anal Bioanal Chem 397:2939-47

Showing the most recent 10 out of 45 publications