Abstract

Retinal cone photoreceptor outer segments will be studied biochemically to isolate and characterize the fucose-containing molecule which is present in cone outer segments but absent in rod outer segments of certain species and to determine if it represents a cone visual pigment. The parameters of shedding of cone outer segment tips will be characterized using electron microscopic and radioautographic techniques. The morphologic pattern and time-course of membrane renewal and turnover in cone outer segments will be documented using staining, radioautographic and freeze fracture techniques at the electron microscope level. Rod and cone outer segment renewal will be studied in the cat retina following photoreceptor damage in experimental commotio retinae and the pattern of breakdown of the blood retinal barrier following this injury will be examined. Early stages of hereditary glaucoma in rabbits will be examined with respect to fine structure and axonal transport alterations in the optic nerve head. The relative contribution of mechanical factors in the etiology of optic nerve head damage in this model for hereditary glaucoma will be tested and the deleterious effects of transport alterations on the ganglion cells of the retina will be documented.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY001311-12
Application #
3255863
Study Section
(VID)
Project Start
1977-02-01
Project End
1987-01-31
Budget Start
1985-02-01
Budget End
1986-01-31
Support Year
12
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of Washington
Department
Type
Schools of Medicine
DUNS #
135646524
City
Seattle
State
WA
Country
United States
Zip Code
98195

  Publications

Wang, T; Steel, G; Milam, A H et al. (2000) Correction of ornithine accumulation prevents retinal degeneration in a mouse model of gyrate atrophy of the choroid and retina. Proc Natl Acad Sci U S A 97:1224-9
Lei, B; Bush, R A; Milam, A H et al. (2000) Human melanoma-associated retinopathy (MAR) antibodies alter the retinal ON-response of the monkey ERG in vivo. Invest Ophthalmol Vis Sci 41:262-6
Fariss, R N; Li, Z Y; Milam, A H (2000) Abnormalities in rod photoreceptors, amacrine cells, and horizontal cells in human retinas with retinitis pigmentosa. Am J Ophthalmol 129:215-23
Humayun, M S; Prince, M; de Juan Jr, E et al. (1999) Morphometric analysis of the extramacular retina from postmortem eyes with retinitis pigmentosa. Invest Ophthalmol Vis Sci 40:143-8
Potter, M J; Thirkill, C E; Dam, O M et al. (1999) Clinical and immunocytochemical findings in a case of melanoma-associated retinopathy. Ophthalmology 106:2121-5
Cideciyan, A V; Hood, D C; Huang, Y et al. (1998) Disease sequence from mutant rhodopsin allele to rod and cone photoreceptor degeneration in man. Proc Natl Acad Sci U S A 95:7103-8
Xiao, M; Sastry, S M; Li, Z Y et al. (1998) Effects of retinal laser photocoagulation on photoreceptor basic fibroblast growth factor and survival. Invest Ophthalmol Vis Sci 39:618-30
Gregory-Evans, K; Fariss, R N; Possin, D E et al. (1998) Abnormal cone synapses in human cone-rod dystrophy. Ophthalmology 105:2306-12
Milam, A H; Li, Z Y; Fariss, R N (1998) Histopathology of the human retina in retinitis pigmentosa. Prog Retin Eye Res 17:175-205
Fariss, R N; Apte, S S; Luthert, P J et al. (1998) Accumulation of tissue inhibitor of metalloproteinases-3 in human eyes with Sorsby's fundus dystrophy or retinitis pigmentosa. Br J Ophthalmol 82:1329-34

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