Abstract

The overall objective is to learn the influence of metabolic processes on aqueous humor outflow, to study factors that alter outflow resistance, and to identify mechanisms of obstruction to outflow in certain secondary glaucomas such as pigmentary glaucoma. Enucleated monkey, calf and human eyes will be perfused via the anterior chamber by a quantitative constant pressure technique in order to determine whether vital metabolic processes in the trabecular meshwork or endothelial wall of Schlemm's canal can influence the facility of aqueous outlfow. The effects of sulfhydryl compounds will be determined. Also, the effects of ascorbic acid and enzymes such as elastase, plasmin, and pectinase on aqueous outflow will be investigated. An attempt will be made to create an experimental model of chronic anterior pigmentary dispersion and pigmentary glaucoma in living monkeys. Morphological correlations will be sought by means of electron microscopy.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY001894-10
Application #
3256288
Study Section
Visual Sciences A Study Section (VISA)
Project Start
1977-04-01
Project End
1988-03-31
Budget Start
1986-04-01
Budget End
1987-03-31
Support Year
10
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
Massachusetts Eye and Ear Infirmary
Department
Type
DUNS #
073825945
City
Boston
State
MA
Country
United States
Zip Code
02114

  Publications

Gonzalez, Pedro; Li, Guorng; Qiu, Jianming et al. (2014) Role of microRNAs in the trabecular meshwork. J Ocul Pharmacol Ther 30:128-37
Huang, Wei; Li, Guorong; Qiu, Jianming et al. (2013) Protective effects of resveratrol in experimental retinal detachment. PLoS One 8:e75735
Luna, Coralia; Li, Guorong; Huang, Jianyong et al. (2012) Regulation of trabecular meshwork cell contraction and intraocular pressure by miR-200c. PLoS One 7:e51688
Wu, Jing; Li, Guorong; Luna, Coralia et al. (2012) Endogenous production of extracellular adenosine by trabecular meshwork cells: potential role in outflow regulation. Invest Ophthalmol Vis Sci 53:7142-8
Luna, Coralia; Li, Guorong; Qiu, Jianming et al. (2011) Cross-talk between miR-29 and transforming growth factor-betas in trabecular meshwork cells. Invest Ophthalmol Vis Sci 52:3567-72
Luna, Coralia; Li, Guorong; Qiu, Jianming et al. (2011) MicroRNA-24 regulates the processing of latent TGFýý1 during cyclic mechanical stress in human trabecular meshwork cells through direct targeting of FURIN. J Cell Physiol 226:1407-14
Li, Guorong; Luna, Coralia; Qiu, Jianming et al. (2011) Role of miR-204 in the regulation of apoptosis, endoplasmic reticulum stress response, and inflammation in human trabecular meshwork cells. Invest Ophthalmol Vis Sci 52:2999-3007
Li, Guorong; Luna, Coralia; Navarro, Iris D et al. (2011) Resveratrol prevention of oxidative stress damage to lens epithelial cell cultures is mediated by forkhead box O activity. Invest Ophthalmol Vis Sci 52:4395-401
Kumar, Janardan; Epstein, David L (2011) Rho GTPase-mediated cytoskeletal organization in Schlemm's canal cells play a critical role in the regulation of aqueous humor outflow facility. J Cell Biochem 112:600-6
Li, Guorong; Luna, Coralia; Qiu, Jianming et al. (2010) Targeting of integrin beta1 and kinesin 2alpha by microRNA 183. J Biol Chem 285:5461-71

Showing the most recent 10 out of 97 publications