The objectives of this research program are to increase our understanding of the morphologic and functional features of normal and abnormal human corneal endothelium and to develop a method for long-term corneal preservation. Because of biologic differences between species, human eyes will be studied whenever possible. Clinical specular microscopy and ocular anterior segment fluorophotometry will be used to study corneal endothelial abnormalities in two groups of patients. First, patients with long- standing Type I diabetes mellitus will be examined for abnormalities in endothelial function; diabetic corneal endothelial cells have been shown previously to have morphologic abnormalities that respond to treatment. Second, the influence of sensory innervation upon corneal hydration in cold ambient air will be investigated in patients with unilateral, surgically- induced corneal anesthesia. Endothelial cell survival after corneal transplantation will be test in patients receiving corneas preserved by new short-term methods. Specular microscopy will be performed preoperatively and at intervals for ten years postoperatively to monitor chronic endothelial survival and morphologic changes in 862 patients who have undergone cataract extraction and 500 patients who have undergone corneal transplantation. Finally, a new method of permanent corneal storage at low temperatures by vitrification will be developed for human corneas and tested by keratoplasty, first in cats and then in humans. Such a method will facilitate the distribution of donor corneas, allow matching of tissue types if necessary, and increase the supply of suitable donor corneas by preventing their loss for logistical reasons.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY002037-13
Application #
3256436
Study Section
Visual Sciences A Study Section (VISA)
Project Start
1978-08-01
Project End
1994-11-30
Budget Start
1990-12-01
Budget End
1991-11-30
Support Year
13
Fiscal Year
1991
Total Cost
Indirect Cost
Name
Mayo Clinic, Rochester
Department
Type
DUNS #
City
Rochester
State
MN
Country
United States
Zip Code
55905
McLaren, Jay W; Bourne, William M; Maguire, Leo J et al. (2015) Changes in Keratocyte Density and Visual Function Five Years After Laser In Situ Keratomileusis: Femtosecond Laser Versus Mechanical Microkeratome. Am J Ophthalmol 160:163-70
Klingler, Kyle N; McLaren, Jay W; Bourne, William M et al. (2012) Corneal endothelial cell changes 5 years after laser in situ keratomileusis: femtosecond laser versus mechanical microkeratome. J Cataract Refract Surg 38:2125-30
Ahuja, Yachna; Baratz, Keith H; McLaren, Jay W et al. (2012) Decreased corneal sensitivity and abnormal corneal nerves in Fuchs endothelial dystrophy. Cornea 31:1257-63
Calvo, Ramón; McLaren, Jay W; Hodge, David O et al. (2010) Corneal aberrations and visual acuity after laser in situ keratomileusis: femtosecond laser versus mechanical microkeratome. Am J Ophthalmol 149:785-93
McLaren, Jay W; Bourne, William M; Patel, Sanjay V (2010) Automated assessment of keratocyte density in stromal images from the ConfoScan 4 confocal microscope. Invest Ophthalmol Vis Sci 51:1918-26
Patel, Sanjay V; Diehl, Nancy N; Hodge, David O et al. (2010) Donor risk factors for graft failure in a 20-year study of penetrating keratoplasty. Arch Ophthalmol 128:418-25
Patel, Sanjay V; McLaren, Jay W; Kittleson, Katrina M et al. (2010) Subbasal nerve density and corneal sensitivity after laser in situ keratomileusis: femtosecond laser vs mechanical microkeratome. Arch Ophthalmol 128:1413-9
McLaren, Jay W; Bourne, William M; Patel, Sanjay V (2010) Standardization of corneal haze measurement in confocal microscopy. Invest Ophthalmol Vis Sci 51:5610-6
Patel, Sangita P; Bourne, William M (2009) Corneal endothelial cell proliferation: a function of cell density. Invest Ophthalmol Vis Sci 50:2742-6
Patel, Sanjay V; Bourne, William M (2009) Corneal endothelial cell loss 9 years after excimer laser keratorefractive surgery. Arch Ophthalmol 127:1423-7

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