Abstract

The objective of the proposed research is the study of neuropeptide containing amacrine cells within the turtle retina. I will use indirect fluorescence and peroxidase immunocytochemical techniques at the light microscopic level to determine the following for each type of neuropeptide containing amacrine cell studied: amacrine cell density and distribution within the retina; the shape of amacrine cell dendritic arbors and cell bodies; and the distribution of amacrine dendrites within subdivisions of the inner plexiform layer. A combination of immunoperoxidase and Golgi techniques will be used in ultrastructural studies to determine the kinds of retinal neurons amacrine cells synaptically contact, and in which subdivisions of the inner plexiform layer these contacts are made. I will also help determine the subcellular storage sites of neuropeptides within retinal amacrine cells and whether or not neuropeptides can co-occur with other neurotransmitters. My studies will expand our knowledge of the role of neuropeptides and amacrine cells in visual processing and provide a sound morphological basis for biochemical and physiological studies of other workers.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY004785-11
Application #
3259302
Study Section
Visual Sciences A Study Section (VISA)
Project Start
1982-08-01
Project End
1994-07-31
Budget Start
1992-08-01
Budget End
1993-07-31
Support Year
11
Fiscal Year
1992
Total Cost
Indirect Cost

  Institution

Name
Boston University
Department
Type
Schools of Arts and Sciences
DUNS #
604483045
City
Boston
State
MA
Country
United States
Zip Code
02118

  Publications

Blom, Jan; Giove, Tom; Deshpande, Monika et al. (2012) Characterization of nitric oxide signaling pathways in the mouse retina. J Comp Neurol 520:4204-17
Blom, Jan; Giove, Thomas J; Pong, Winnie W et al. (2012) Evidence for a functional adrenomedullin signaling pathway in the mouse retina. Mol Vis 18:1339-53
Giove, Thomas J; Sena-Esteves, Miguel; Eldred, William D (2010) Transduction of the inner mouse retina using AAVrh8 and AAVrh10 via intravitreal injection. Exp Eye Res 91:652-9
Giove, Thomas J; Deshpande, Monika M; Eldred, William D (2009) Identification of alternate transcripts of neuronal nitric oxide synthase in the mouse retina. J Neurosci Res 87:3134-42
Blom, Jan J; Blute, Todd A; Eldred, William D (2009) Functional localization of the nitric oxide/cGMP pathway in the salamander retina. Vis Neurosci 26:275-86
Giove, Thomas J; Deshpande, Monika M; Gagen, Christine S et al. (2009) Increased neuronal nitric oxide synthase activity in retinal neurons in early diabetic retinopathy. Mol Vis 15:2249-58
Pong, Winnie W; Eldred, William D (2009) Interactions of the gaseous neuromodulators nitric oxide, carbon monoxide, and hydrogen sulfide in the salamander retina. J Neurosci Res 87:2356-64
Xie, Z; Adamowicz, W O; Eldred, W D et al. (2006) Cellular and subcellular localization of PDE10A, a striatum-enriched phosphodiesterase. Neuroscience 139:597-607
Yu, Dou; Eldred, William D (2005) Nitric oxide stimulates gamma-aminobutyric acid release and inhibits glycine release in retina. J Comp Neurol 483:278-91
Eldred, William D; Blute, Todd A (2005) Imaging of nitric oxide in the retina. Vision Res 45:3469-86

Showing the most recent 10 out of 11 publications