The goal of this study is to define the function of metabotropic glutamate and GABA receptors in the communication link between bipolar and ganglion cells. Recent studies have described and identified several types of metabotropic glutamate and GABA receptors on retinal ganglion cells. The investigator has found that these receptors also modulate bipolar cell transmitter release. The proposed research addresses the mechanisms and the ramifications of their actions on the light response properties of the IPL.
One aim will be to determine if bipolar cell transmitter release is controlled by two metabotropic GABA receptor subtypes, one activated by baclofen and the other by cis-aminocrotonic acid. These receptors may regulate different aspects of bipolar cell transmitter release and act under different states of retinal adaptation. Metabotropic glutamate receptors may function as negative-feedback autoreceptors that monitor bipolar cell transmitter release. Subtypes of metabotropic glutamate receptors, localized to each side of the bipolar cell dyad synapse, may differentially modulate transmitter output to amacrine and ganglion cells. Amacrine cells possess both metabotropic GABA and glutamate receptors. The function of these receptors, both as presynaptic autoreceptors and postsynaptic receptors, will be investigated, which will extend our knowledge of synaptic signaling and information processing in the retina.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY005725-20
Application #
6627006
Study Section
Visual Sciences C Study Section (VISC)
Program Officer
Mariani, Andrew P
Project Start
1984-08-01
Project End
2004-12-31
Budget Start
2003-01-01
Budget End
2003-12-31
Support Year
20
Fiscal Year
2003
Total Cost
$290,968
Indirect Cost
Name
State University of New York at Buffalo
Department
Physiology
Type
Schools of Medicine
DUNS #
038633251
City
Buffalo
State
NY
Country
United States
Zip Code
14260
Garaycochea, Jay; Slaughter, Malcolm M (2016) GABAB receptors enhance excitatory responses in isolated rat retinal ganglion cells. J Physiol 594:5543-54
Wu, Fuguo; Kaczynski, Tadeusz J; Sethuramanujam, Santhosh et al. (2015) Two transcription factors, Pou4f2 and Isl1, are sufficient to specify the retinal ganglion cell fate. Proc Natl Acad Sci U S A 112:E1559-68
Sethuramanujam, Santhosh; Slaughter, Malcolm M (2014) Disinhibitory recruitment of NMDA receptor pathways in retina. J Neurophysiol 112:193-203
Li, Ping; Slaughter, Malcolm M (2012) Gating effects on picrotin block of glycine receptors. Neuroreport 23:1017-20
Song, Yunbo; Slaughter, Malcolm M (2010) GABA(B) receptor feedback regulation of bipolar cell transmitter release. J Physiol 588:4937-49
Duan, Lei; Yang, Jaeyoung; Slaughter, Malcolm M (2009) Caffeine inhibition of ionotropic glycine receptors. J Physiol 587:4063-75
Frolov, R V; Slaughter, M M; Singh, S (2008) Effects of celecoxib on ionic currents and spontaneous firing in rat retinal neurons. Neuroscience 154:1525-32
Shen, W; Slaughter, M M (2001) Multireceptor GABAergic regulation of synaptic communication in amphibian retina. J Physiol 530:55-67
Tian, N; Slaughter, M M (1994) Pharmacological similarity between the retinal APB receptor and the family of metabotropic glutamate receptors. J Neurophysiol 71:2258-68
Tian, N; Slaughter, M M (1994) Pharmacology of the GABAB receptor in amphibian retina. Brain Res 660:267-74

Showing the most recent 10 out of 15 publications