The focus of this project will be to elucidate the mechanism of spectral tuning in the subgroup of short-wave visual pigments. First, the protonation state of the Schiff base nitrogen in the 11-cis retinal chromophore of the ultraviolet, human blue and bovine blue pigments will be determined by 15N-solid state magic angle spinning (MAS) NMR spectroscopy. Second the amino acid residues responsible for spectral tuning within the short-wave group will be identified. Finally the mechanism of spectral tuning in the short-wave subgroup will be compared to that of other subgroups of visual pigments. In particular, attention will be focused on the zebrafish blue pigment because it is a member of the middle-wavelength subgroup of pigments (based on its amino acid sequence) but has a maximum centered in the short wavelength range (419nm). Results from this work will shed light on the mechanisms of spectral tuning among the visual pigments to illuminate the mechanistic basis for the evolutionary development of human color vision.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY009514-12
Application #
6741880
Study Section
Special Emphasis Panel (ZRG1-SSS-R (03))
Program Officer
Mariani, Andrew P
Project Start
1992-05-01
Project End
2006-04-30
Budget Start
2004-05-01
Budget End
2006-04-30
Support Year
12
Fiscal Year
2004
Total Cost
$271,250
Indirect Cost
Name
Brandeis University
Department
Biochemistry
Type
Schools of Arts and Sciences
DUNS #
616845814
City
Waltham
State
MA
Country
United States
Zip Code
02454
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