Primary open-angle glaucoma (POAG) is the second leading cause of blindness in the United States and is commonly associated with elevation of intraocular pressure (IOP) resulting from increased resistance to aqueous humor (AH) outflow through the trabecular meshwork (TM) and Schlemm's canal (SC). Although IOP is considered to be a primary risk factor for open angle glaucoma, the etiological mechanisms responsible for increased resistance to AH outflow are largely unknown. The long-range goal of this application is to identify specific etiological mechanisms underlying increased resistance to AH outflow through the TM and SC, and leverage this knowledge to develop novel and targeted therapies for lowering IOP in glaucoma patients. We recently developed an animal model that exhibits a sustained increase in IOP in response to expression of a constitutively active Rho GTPase (RhoAV14) in the AH outflow pathway. Based on the highly significant and promising preliminary observations derived from this model, we propose a novel hypothesis that ocular hypertension induced by aberrant Rho/Rho kinase signaling activity in the trabecular outflow pathway is mechanistically linked to an enhanced endothelial to mesenchymal transition of TM and SC cells into matrix producing myofibroblast-like cells, the activation of which results in extracellular matrix (ECM) deposition and changes in juxtacanalicular connective tissue (JCT) architecture, leading to increased resistance to AH outflow. This central hypothesis is supported by our findings of scarring of the JCT, ECM accumulation and expression of myofibroblast-specific markers in the trabecular pathway of RhoAV14-induced ocular hypertensive rat eyes and in human POAG donor eyes. We propose to investigate this novel hypothesis under three specific aims: 1. Establish the role of Rho/Rho kinase signaling in driving the endothelial to mesenchymal transition (EndMT) of TM and SC cells into matrix-producing myofibroblast-like cells, upon exposure to mechanical strain and physiologically relevant factors (TGF-?, autotaxin/LPA axis, connective tissue growth factor) associated with glaucoma;2. Verify the mechanistic link between ocular hypertension in the RhoAV14 rat model, increased EndMT of TM and SC cells into myofibroblast-like cells, structural alterations within AH outflow pathway tissues, and increased resistance to AH outflow, and 3. Evaluate the validity of the prediction that the efficacy of Rho kinase inhibitors to increase AH outflow in ocular hypertensive glaucomatous eyes is linked partly to suppression of ECM deposition and fibrogenic effects of activated myofibroblasts in AH outflow pathway, using the RhoAV14 ocular hypertensive rat as a model system. Exploration of these studies is expected to identify the specific etiological mechanisms involved in increased resistance to AH outflow in glaucoma subjects and enhance our mechanistic understanding of how certain physiological factors and Rho kinase inhibitors influence AH outflow and IOP in glaucoma patients.

Public Health Relevance

Primary open-angle glaucoma is the second leading cause of blindness in the United States and is commonly associated with elevated intraocular pressure (IOP) due to diminished aqueous humor drainage. Developing effective therapies for increased IOP in glaucoma patients requires identification of the specific etiological mechanisms that underlie resistance to aqueous humor (AH) outflow. In this application we propose to investigate the novel hypothesis that an aberrant endothelial to mesenchymal transition (EndMT) of trabecular meshwork and Schlemm's canal cells into matrix producing activated myofibroblast-like cells represents a critical causative mechanism accounting for impaired AH outflow in glaucoma patients. It is anticipated that completion of this research will provide significant insights into our understanding of both physiological and pathological regulation of aqueous humor outflow resistance in normal and ocular hypertensive glaucoma eyes, respectively, and have a direct impact on development of novel strategies for glaucoma treatment.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY018590-06
Application #
8657438
Study Section
(DPVS)
Program Officer
Chin, Hemin R
Project Start
2008-01-01
Project End
2018-04-30
Budget Start
2014-05-01
Budget End
2015-04-30
Support Year
6
Fiscal Year
2014
Total Cost
Indirect Cost
Name
Duke University
Department
Ophthalmology
Type
Schools of Medicine
DUNS #
City
Durham
State
NC
Country
United States
Zip Code
27705
Ho, Leona T Y; Skiba, Nikolai; Ullmer, Christoph et al. (2018) Lysophosphatidic Acid Induces ECM Production via Activation of the Mechanosensitive YAP/TAZ Transcriptional Pathway in Trabecular Meshwork Cells. Invest Ophthalmol Vis Sci 59:1969-1984
Maddala, Rupalatha; Skiba, Nikolai P; Rao, Ponugoti Vasantha (2017) Vertebrate Lonesome Kinase Regulated Extracellular Matrix Protein Phosphorylation, Cell Shape, and Adhesion in Trabecular Meshwork Cells. J Cell Physiol 232:2447-2460
Rao, Ponugoti Vasantha; Pattabiraman, Padmanabhan P; Kopczynski, Casey (2017) Role of the Rho GTPase/Rho kinase signaling pathway in pathogenesis and treatment of glaucoma: Bench to bedside research. Exp Eye Res 158:23-32
Muralidharan, Arumugam Ramachandran; Maddala, Rupalatha; Skiba, Nikolai P et al. (2016) Growth Differentiation Factor-15-Induced Contractile Activity and Extracellular Matrix Production in Human Trabecular Meshwork Cells. Invest Ophthalmol Vis Sci 57:6482-6495
Rao, Ponugoti Vasantha; Maddala, Rupalatha (2016) Ankyrin-B in lens architecture and biomechanics: Just not tethering but more. Bioarchitecture 6:39-45
Maddala, Rupalatha; Walters, Mark; Brophy, Peter J et al. (2016) Ankyrin-B directs membrane tethering of periaxin and is required for maintenance of lens fiber cell hexagonal shape and mechanics. Am J Physiol Cell Physiol 310:C115-26
Meekins, Landon C; Rosado-Adames, Noel; Maddala, Rupalatha et al. (2016) Corneal Endothelial Cell Migration and Proliferation Enhanced by Rho Kinase (ROCK) Inhibitors in In Vitro and In Vivo Models. Invest Ophthalmol Vis Sci 57:6731-6738
Pattabiraman, Padmanabhan P; Inoue, Toshihiro; Rao, P Vasantha (2015) Elevated intraocular pressure induces Rho GTPase mediated contractile signaling in the trabecular meshwork. Exp Eye Res 136:29-33
Maddala, Rupalatha; Nagendran, Tharkika; Lang, Richard A et al. (2015) Rap1 GTPase is required for mouse lens epithelial maintenance and morphogenesis. Dev Biol 406:74-91
Pattabiraman, Padmanabhan P; Rinkoski, Tommy; Poeschla, Eric et al. (2015) RhoA GTPase-induced ocular hypertension in a rodent model is associated with increased fibrogenic activity in the trabecular meshwork. Am J Pathol 185:496-512

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