Abstract

The long term objective of the proposed work is to further develop and exploit the potential of field-flow fractionation (FFF) as a tool of wide applicability in the biological sciences. The specific development work aimed at this goal will consist mainly of basic theoretical and experimental studies of FFF performance and optimization, the launching of a program to exploit the multidimensional capabilities of FFF, and more limited projects in applications and basic theory. Work will be undertaken to improve performance through the optimization of channel geometry and dimensions, the further development of split inlet and outlet systems, the optimization of hydrodynamic relaxation, and the fuller utilization of digital methods for data interpretation and instrument control. Programmed FFF will be further optimized. A multidimensional FFF system will be developed by linking together sedimentation FFF and flow FFF systems with the objective of more fully characterizing complex colloids such as those of biological origin. Some applications will be undertaken and further work will be done on broader theoretical and methodological issues in analytical separations.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM010851-36
Application #
3268165
Study Section
Metallobiochemistry Study Section (BMT)
Project Start
1979-02-01
Project End
1996-03-31
Budget Start
1993-04-01
Budget End
1994-03-31
Support Year
36
Fiscal Year
1993
Total Cost
Indirect Cost

  Institution

Name
University of Utah
Department
Type
Schools of Arts and Sciences
DUNS #
City
Salt Lake City
State
UT
Country
United States
Zip Code
84112

  Publications

Benincasa, M A; Caldwell, K D (2001) Flow field-flow fractionation of poly(ethylene oxide): effect of carrier ionic strength and composition. J Chromatogr A 925:159-69
Tong, X; Caldwell, K D (1999) Computer simulation of particle separation based on non-equilibrium swelling. J Chromatogr A 831:51-62
Vauthier, J C; Williams, P S (1998) Numerical simulation of band-broadening during hydrodynamic relaxation in frit-inlet field-flow fractionation channels. J Chromatogr A 805:149-60
Jensen, K D; Williams, S K; Giddings, J C (1996) High-speed particle separation and steric inversion in thin flow field-flow fractionation channels. J Chromatogr A 746:137-45
Giddings, J C (1995) Sample dimensionality: a predictor of order-disorder in component peak distribution in multidimensional separation. J Chromatogr A 703:3-15
Giddings, J C; Xu, Y; Myers, M N (1994) Enhancement of performance in sedimentation field-flow fractionation by temperature elevation. Anal Chem 66:3047-53
Williams, P S; Giddings, J C (1994) Theory of field-programmed field-flow fractionation with corrections for steric effects. Anal Chem 66:4215-28
Giddings, J C (1993) Field-flow fractionation: analysis of macromolecular, colloidal, and particulate materials. Science 260:1456-65
Barman, B N; Ashwood, E R; Giddings, J C (1993) Separation and size distribution of red blood cells of diverse size, shape, and origin by flow/hyperlayer field-flow fractionation. Anal Biochem 212:35-42
Moon, M H; Giddings, J C (1993) Size distribution of liposomes by flow field-flow fractionation. J Pharm Biomed Anal 11:911-20

Showing the most recent 10 out of 36 publications