The objective of this research is to broaden our understanding of mammalian genetics through the formal genetic analysis of the mouse. The major focus of the research is on the development and use of recombinant inbred (RI) mouse strains for the analysis of both traits. These RI strains are widely used by other investigators for mapping polymorphic loci and for analyzing complex traits such as resistance to microbial challenge. DNA restriction fragment variants associated with selected genes will be identified and mapped using these strains. As more markers are typed in the system the RI sets become an increasingly powerful genetic tool. By mapping selected genes through these DNA variants it may be possible to identify mouse mutations at the probe loci. This approach has led to the discovery of close linkage between one such mutation, called congenital goiter, and the gene encoding thyroglobulin. The molecular basis for this mutation will be examined by the analysis of the thyroglobulin gene, mRNA, and protein in mutant and normal mice. In addition, the feasibility of deletion mapping the murine genome using DNA markers will be tested.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM018684-17
Application #
3269355
Study Section
Mammalian Genetics Study Section (MGN)
Project Start
1977-07-01
Project End
1992-06-30
Budget Start
1988-07-01
Budget End
1989-06-30
Support Year
17
Fiscal Year
1988
Total Cost
Indirect Cost
Name
Jackson Laboratory
Department
Type
DUNS #
042140483
City
Bar Harbor
State
ME
Country
United States
Zip Code
04609
Traina-Dorge, V L; Carr, J K; Bailey-Wilson, J E et al. (1985) Cellular genes in the mouse regulate in trans the expression of endogenous mouse mammary tumor viruses. Genetics 111:597-615