Abstract

A search will be conducted for conditional yeast mutants deficient in the synthesis and regulation of important membrane lipids. These mutants will be characterized both genetically and biochemically. Rates and timing of synthesis of individual lipids, during the mitotic cell cycle will be determined in both wild type and mutant yeast. The dependence of basic cellular processes, such as macromolecular synthesis and cell division on lipid biosynthesis will be assessed and roles of individual lipids studied. Since lipids are important components of biomembranes, it is hoped that the information gained will give some insight into the timing and control of membrane biosynthesis during cell division and into the structural and functional roles of lipids in membranes. Very little is presently known about the molecular organization of biological membranes or about membrane synthesis. Because membranes are involved in a multiplicity of cellular processes including the transport of substances into and out of cells, they must be expected to play an important part in both normal and abnormal cell growth and differentiation.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM019629-14
Application #
3269705
Study Section
Genetics Study Section (GEN)
Project Start
1978-09-01
Project End
1986-08-31
Budget Start
1985-09-01
Budget End
1986-08-31
Support Year
14
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Albert Einstein College of Medicine
Department
Type
Schools of Medicine
DUNS #
009095365
City
Bronx
State
NY
Country
United States
Zip Code
10461

  Publications

Gaspar, Maria L; Chang, Yu-Fang; Jesch, Stephen A et al. (2017) Interaction between repressor Opi1p and ER membrane protein Scs2p facilitates transit of phosphatidic acid from the ER to mitochondria and is essential for INO1 gene expression in the presence of choline. J Biol Chem 292:18713-18728
Mondo, Stephen J; Lastovetsky, Olga A; Gaspar, Maria L et al. (2017) Bacterial endosymbionts influence host sexuality and reveal reproductive genes of early divergent fungi. Nat Commun 8:1843
Lastovetsky, Olga A; Gaspar, Maria L; Mondo, Stephen J et al. (2016) Lipid metabolic changes in an early divergent fungus govern the establishment of a mutualistic symbiosis with endobacteria. Proc Natl Acad Sci U S A 113:15102-15107
Barneda, David; Planas-Iglesias, Joan; Gaspar, Maria L et al. (2015) The brown adipocyte protein CIDEA promotes lipid droplet fusion via a phosphatidic acid-binding amphipathic helix. Elife 4:e07485
Han, Sungwon; Binns, Derk D; Chang, Yu-Fang et al. (2015) Dissecting seipin function: the localized accumulation of phosphatidic acid at ER/LD junctions in the absence of seipin is suppressed by Sei1p(?Nterm) only in combination with Ldb16p. BMC Cell Biol 16:29
Henry, Susan A; Gaspar, Maria L; Jesch, Stephen A (2014) The response to inositol: regulation of glycerolipid metabolism and stress response signaling in yeast. Chem Phys Lipids 180:23-43
Hofbauer, Harald F; Schopf, Florian H; Schleifer, Hannes et al. (2014) Regulation of gene expression through a transcriptional repressor that senses acyl-chain length in membrane phospholipids. Dev Cell 29:729-39
Lee, Sojin; Gaspar, Maria L; Aregullin, Manuel A et al. (2013) Activation of protein kinase C-mitogen-activated protein kinase signaling in response to inositol starvation triggers Sir2p-dependent telomeric silencing in yeast. J Biol Chem 288:27861-71
Aung, Hnin W; Henry, Susan A; Walker, Larry P (2013) Revising the Representation of Fatty Acid, Glycerolipid, and Glycerophospholipid Metabolism in the Consensus Model of Yeast Metabolism. Ind Biotechnol (New Rochelle N Y) 9:215-228
Henry, Susan A; Kohlwein, Sepp D; Carman, George M (2012) Metabolism and regulation of glycerolipids in the yeast Saccharomyces cerevisiae. Genetics 190:317-49

Showing the most recent 10 out of 17 publications