Abstract

The application has three overall goals; first, the mechanism of mating type switching in baker's yeast will be examined, and in particular the molecular basis of donor preference, second, the molecular details of double strand break (DSB) induced recombination will be studied and third, the role of genes in replication, repair and recombination in DSB-induced recombination will be examined. Also the effect of chromatin on mating type switching and other DSB-induced events will be studied.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM020056-25
Application #
2444453
Study Section
Genetics Study Section (GEN)
Project Start
1976-01-01
Project End
1999-06-30
Budget Start
1997-07-01
Budget End
1998-06-30
Support Year
25
Fiscal Year
1997
Total Cost
Indirect Cost

  Institution

Name
Brandeis University
Department
Type
Organized Research Units
DUNS #
616845814
City
Waltham
State
MA
Country
United States
Zip Code
02454

  Publications

Gallagher, Danielle N; Haber, James E (2018) Repair of a Site-Specific DNA Cleavage: Old-School Lessons for Cas9-Mediated Gene Editing. ACS Chem Biol 13:397-405
Lemos, Brenda R; Kaplan, Adam C; Bae, Ji Eun et al. (2018) CRISPR/Cas9 cleavages in budding yeast reveal templated insertions and strand-specific insertion/deletion profiles. Proc Natl Acad Sci U S A 115:E2040-E2047
Haber, James E (2018) DNA Repair: The Search for Homology. Bioessays 40:e1700229
Eapen, Vinay V; Waterman, David P; Bernard, Amélie et al. (2017) A pathway of targeted autophagy is induced by DNA damage in budding yeast. Proc Natl Acad Sci U S A 114:E1158-E1167
Mehta, Anuja; Beach, Annette; Haber, James E (2017) Homology Requirements and Competition between Gene Conversion and Break-Induced Replication during Double-Strand Break Repair. Mol Cell 65:515-526.e3
Tsabar, Michael; Haase, Julian; Harrison, Benjamin et al. (2016) A Cohesin-Based Partitioning Mechanism Revealed upon Transcriptional Inactivation of Centromere. PLoS Genet 12:e1006021
Tsabar, Michael; Hicks, Wade M; Tsaponina, Olga et al. (2016) Re-establishment of nucleosome occupancy during double-strand break repair in budding yeast. DNA Repair (Amst) 47:21-29
Lee, Cheng-Sheng; Wang, Ruoxi W; Chang, Hsiao-Han et al. (2016) Chromosome position determines the success of double-strand break repair. Proc Natl Acad Sci U S A 113:E146-54
Av?aro?lu, Bar??; Bronk, Gabriel; Li, Kevin et al. (2016) Chromosome-refolding model of mating-type switching in yeast. Proc Natl Acad Sci U S A 113:E6929-E6938
Tsabar, Michael; Waterman, David P; Aguilar, Fiona et al. (2016) Asf1 facilitates dephosphorylation of Rad53 after DNA double-strand break repair. Genes Dev 30:1211-24

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