Abstract

Dr. David R. Zusman proposes to examine sensory transduction in Myxococcus xanthus by examining the Frz pathway, which is homologous to the Che pathway of the enteric bacteria. The frz genes control cell reversal frequency and are essential for directed cell movement during fruiting body development. This project explores fundamental processes of sensory transduction in a model organisms which may have application to our understanding of similar processes in higher cells. The proposal has 4 basic objectives: 1) Characterize FrzZ, a novel CheY like protein with two phosphoacceptor domains; 2) identify the motor switch components that are thought to interact with FrzE; 3) identify attractant(s) which may be responsible for directing fruiting body formation; 4) determine whether beta-lactamase induces sporulation and determine the mechanism by which the gene is regulated.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM020509-27
Application #
6018452
Study Section
Special Emphasis Panel (ZRG5-TMP (04))
Program Officer
Anderson, James J
Project Start
1976-06-01
Project End
2001-02-28
Budget Start
1999-08-01
Budget End
2001-02-28
Support Year
27
Fiscal Year
1999
Total Cost
Indirect Cost

  Institution

Name
University of California Berkeley
Department
Biochemistry
Type
Schools of Arts and Sciences
DUNS #
094878337
City
Berkeley
State
CA
Country
United States
Zip Code
94704

  Publications

Fu, Guo; Bandaria, Jigar N; Le Gall, Anne Valérie et al. (2018) MotAB-like machinery drives the movement of MreB filaments during bacterial gliding motility. Proc Natl Acad Sci U S A 115:2484-2489
Berleman, James E; Zemla, Marcin; Remis, Jonathan P et al. (2016) Exopolysaccharide microchannels direct bacterial motility and organize multicellular behavior. ISME J 10:2620-2632
Nan, Beiyan; Zusman, David R (2016) Novel mechanisms power bacterial gliding motility. Mol Microbiol 101:186-93
Kaimer, Christine; Zusman, David R (2016) Regulation of cell reversal frequency in Myxococcus xanthus requires the balanced activity of CheY-like domains in FrzE and FrzZ. Mol Microbiol 100:379-95
Nan, Beiyan; Bandaria, Jigar N; Guo, Kathy Y et al. (2015) The polarity of myxobacterial gliding is regulated by direct interactions between the gliding motors and the Ras homolog MglA. Proc Natl Acad Sci U S A 112:E186-93
Nan, Beiyan; McBride, Mark J; Chen, Jing et al. (2014) Bacteria that glide with helical tracks. Curr Biol 24:R169-73
Moine, Audrey; Agrebi, Rym; Espinosa, Leon et al. (2014) Functional organization of a multimodular bacterial chemosensory apparatus. PLoS Genet 10:e1004164
Kaimer, Christine; Zusman, David R (2013) Phosphorylation-dependent localization of the response regulator FrzZ signals cell reversals in Myxococcus xanthus. Mol Microbiol 88:740-53
Nan, Beiyan; Bandaria, Jigar N; Moghtaderi, Amirpasha et al. (2013) Flagella stator homologs function as motors for myxobacterial gliding motility by moving in helical trajectories. Proc Natl Acad Sci U S A 110:E1508-13
Kaimer, Christine; Berleman, James E; Zusman, David R (2012) Chemosensory signaling controls motility and subcellular polarity in Myxococcus xanthus. Curr Opin Microbiol 15:751-7

Showing the most recent 10 out of 24 publications