Abstract

The goal of the proposed study is the expansion of the scope of high performance liquid chromatography to the rapid separation of complex biological mixtures including those of biopolymers. The approach involves the development of novel microparticulate stationary phases and eluent systems as well as the investigation of the pertinent chromatographic parameters both experimentally and theoretically. The results are applied to the development of advanced methods for biochemical analysis as well as preparative scale separation of proteins, peptides and nucleic acids and their fragments.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM020993-12
Application #
3270205
Study Section
Metallobiochemistry Study Section (BMT)
Project Start
1978-02-01
Project End
1988-02-29
Budget Start
1986-03-01
Budget End
1987-02-28
Support Year
12
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
Yale University
Department
Type
Schools of Arts and Sciences
DUNS #
082359691
City
New Haven
State
CT
Country
United States
Zip Code
06520

  Publications

Shi, Yang; Xiang, Rong; Horvath, Csaba et al. (2005) Quantitative analysis of membrane proteins from breast cancer cell lines BT474 and MCF7 using multistep solid phase mass tagging and 2D LC/MS. J Proteome Res 4:1427-33
Shi, Yang; Xiang, Rong; Horvath, Csaba et al. (2005) Design and synthesis of a solid-phase fluorescent mass tag. J Sep Sci 28:1812-7
Li, Yan; Chen, Yuan; Xiang, Rong et al. (2005) Incorporation of single-wall carbon nanotubes into an organic polymer monolithic stationary phase for mu-HPLC and capillary electrochromatography. Anal Chem 77:1398-406
Shi, Yang; Hill, Jane; Xiang, Rong et al. (2005) Multistep mass tagging coupled with 2D LC-MS--an approach to increasing the number of identified proteins. J Biomol Tech 16:190-6
Rathore, Anurag S; Li, Yan; Wilkins, James (2005) Use of electrokinetic measurements for characterization of columns used in capillary electrochromatography. J Chromatogr A 1079:299-306
Shi, Yang; Xiang, Rong; Crawford, Janet K et al. (2004) A simple solid phase mass tagging approach for quantitative proteomics. J Proteome Res 3:104-11
Shi, Yang; Xiang, Rong; Horvath, Csaba et al. (2004) The role of liquid chromatography in proteomics. J Chromatogr A 1053:27-36
Li, Yan; Zhang, Jian; Xiang, Rong et al. (2004) Preparation and characterization of alkylated polymethacrylate monolithic columns for micro-HPLC of proteins. J Sep Sci 27:1467-74
Li, Yan; Xiang, Rong; Wilkins, James A et al. (2004) Capillary electrochromatography of peptides and proteins. Electrophoresis 25:2242-56
Li, Yan; Xiang, Rong; Horvath, Csaba et al. (2004) Capillary electrochromatography of peptides on a neutral porous monolith with annular electroosmotic flow generation. Electrophoresis 25:545-53

Showing the most recent 10 out of 88 publications