Abstract

The proposed research is designed to integrate stereological and biochemical data in order that changes in hepatocytic membranes can be described by structure function equations. These equations will be used to characterize heterogeneous distributions of marker enzymes at single and at multiple organelle locations and to locate these heterogeneities within specific subpopulations of structurally and functionally similar hepatocytes. chemical models will be used to induce several different changes in hepatocytic membranes and these effects will be evaluated using a new data intensive integrated systems approach.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM022759-08
Application #
3271306
Study Section
Molecular Cytology Study Section (CTY)
Project Start
1976-04-01
Project End
1987-05-31
Budget Start
1985-05-01
Budget End
1987-05-31
Support Year
8
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of Washington
Department
Type
Schools of Medicine
DUNS #
135646524
City
Seattle
State
WA
Country
United States
Zip Code
98195

  Publications

Bertram, J F; Bolender, R P (1990) Counting cells with stereology: random versus serial sectioning. J Electron Microsc Tech 14:32-8
Gittes, F (1990) Estimating mean particle volume and number from random sections by sampling profile boundaries. J Microsc 158:1-18
Pentcheff, N D; Bolender, R P (1985) PCS System I: point counting stereology programs for cell biology. Comput Methods Programs Biomed 20:173-87