Abstract

We will investigate the light regulation of the expression of two nuclear coded genes in Lemna. These genes are those for the small subunit of ribulose 1,5 bisphosphate carboxylase (RuBP Case) and the chlorophyll (chl) a/b protein. We will select cloned hybridization probes for these genes in order to study the light regulation in detail. We have shown that the level of translatable mRNAs for these two proteins can be regulated by phytochrome. We hope to demonstrate which step--transcription, post-transcriptional processing, or degradation of the mRNAs--is actually affected by phytochrome action. In addition, we shall investigate a second light-regulated step necessary for the effective synthesis of the chl a/b protein.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM023167-11
Application #
3271523
Study Section
Molecular Cytology Study Section (CTY)
Project Start
1976-06-01
Project End
1987-07-31
Budget Start
1986-08-01
Budget End
1987-07-31
Support Year
11
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
University of California Los Angeles
Department
Type
Schools of Arts and Sciences
DUNS #
119132785
City
Los Angeles
State
CA
Country
United States
Zip Code
90095

  Publications

Liu, Hongtao; Wang, Qin; Liu, Yawen et al. (2013) Arabidopsis CRY2 and ZTL mediate blue-light regulation of the transcription factor CIB1 by distinct mechanisms. Proc Natl Acad Sci U S A 110:17582-7
Lu, Sheen X; Webb, Candace J; Knowles, Stephen M et al. (2012) CCA1 and ELF3 Interact in the control of hypocotyl length and flowering time in Arabidopsis. Plant Physiol 158:1079-88
Lu, Sheen X; Liu, Hongtao; Knowles, Stephen M et al. (2011) A role for protein kinase casein kinase2 *-subunits in the Arabidopsis circadian clock. Plant Physiol 157:1537-45
Lu, Sheen X; Knowles, Stephen M; Webb, Candace J et al. (2011) The Jumonji C domain-containing protein JMJ30 regulates period length in the Arabidopsis circadian clock. Plant Physiol 155:906-15
Bu, Qingyun; Zhu, Ling; Dennis, Michael D et al. (2011) Phosphorylation by CK2 enhances the rapid light-induced degradation of phytochrome interacting factor 1 in Arabidopsis. J Biol Chem 286:12066-74
Lu, Sheen X; Tobin, Elaine M (2011) Chromatin remodeling and the circadian clock: Jumonji C-domain containing proteins. Plant Signal Behav 6:810-4
Lu, Sheen X; Knowles, Stephen M; Andronis, Christos et al. (2009) CIRCADIAN CLOCK ASSOCIATED1 and LATE ELONGATED HYPOCOTYL function synergistically in the circadian clock of Arabidopsis. Plant Physiol 150:834-43
Gardner, Gary; Lin, Chentao; Tobin, Elaine M et al. (2009) Photobiological properties of the inhibition of etiolated Arabidopsis seedling growth by ultraviolet-B irradiation. Plant Cell Environ 32:1573-83
Andronis, Christos; Barak, Simon; Knowles, Stephen M et al. (2008) The clock protein CCA1 and the bZIP transcription factor HY5 physically interact to regulate gene expression in Arabidopsis. Mol Plant 1:58-67
Knowles, Stephen M; Lu, Sheen X; Tobin, Elaine M (2008) Testing time: can ethanol-induced pulses of proposed oscillator components phase shift rhythms in Arabidopsis? J Biol Rhythms 23:463-71

Showing the most recent 10 out of 39 publications