Abstract

This proposal concerns mitotic spindle function in lysed mammalian tissue culture cells. Cells lysed early in anaphase into a mixture containing polyethylene glycol and nonionic detergents will continue to move chromsomes towards the spindles poles and display spindle elongation. This process requires ATP. The cells are permeable to dyes, ions and some proteins. This system is being used to characterize the nucleotide, divalent cation, ionic requirements for chromosome movement. The effects on chromosome movement of adding relevant proteins such as tubulin, dynein, actin and antibodies to various contractile proteins to the lysis medium are also being studied.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM023238-10
Application #
3271547
Study Section
Cellular Biology and Physiology Subcommittee 1 (CBY)
Project Start
1976-05-01
Project End
1987-06-30
Budget Start
1985-07-01
Budget End
1986-06-30
Support Year
10
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of California Berkeley
Department
Type
Schools of Arts and Sciences
DUNS #
094878337
City
Berkeley
State
CA
Country
United States
Zip Code
94704

  Publications

Jin, Ye; Uzawa, Satoru; Cande, W Z (2002) Fission yeast mutants affecting telomere clustering and meiosis-specific spindle pole body integrity. Genetics 160:861-76
Brazer, S C; Williams, H P; Chappell, T G et al. (2000) A fission yeast kinesin affects Golgi membrane recycling. Yeast 16:149-66
Pidoux, A L; Uzawa, S; Perry, P E et al. (2000) Live analysis of lagging chromosomes during anaphase and their effect on spindle elongation rate in fission yeast. J Cell Sci 113 Pt 23:4177-91
Paluh, J L; Nogales, E; Oakley, B R et al. (2000) A mutation in gamma-tubulin alters microtubule dynamics and organization and is synthetically lethal with the kinesin-like protein pkl1p. Mol Biol Cell 11:1225-39
Kaszas, E; Cande, W Z (2000) Phosphorylation of histone H3 is correlated with changes in the maintenance of sister chromatid cohesion during meiosis in maize, rather than the condensation of the chromatin. J Cell Sci 113 ( Pt 18):3217-26
Wein, H; Bass, H W; Cande, W Z (1998) DSK1, a kinesin-related protein involved in anaphase spindle elongation, is a component of a mitotic spindle matrix. Cell Motil Cytoskeleton 41:214-24
Wein, H; Foss, M; Brady, B et al. (1996) DSK1, a novel kinesin-related protein from the diatom Cylindrotheca fusiformis that is involved in anaphase spindle elongation. J Cell Biol 133:595-604
Pidoux, A L; LeDizet, M; Cande, W Z (1996) Fission yeast pkl1 is a kinesin-related protein involved in mitotic spindle function. Mol Biol Cell 7:1639-55
Dawe, R K; Sedat, J W; Agard, D A et al. (1994) Meiotic chromosome pairing in maize is associated with a novel chromatin organization. Cell 76:901-12
Hogan, C J; Stephens, L; Shimizu, T et al. (1992) Physiological evidence for involvement of a kinesin-related protein during anaphase spindle elongation in diatom central spindles. J Cell Biol 119:1277-86

Showing the most recent 10 out of 29 publications