Abstract

Myogenic cells grown in culture retain many of their differentiated, cell surface associated properties. Using our recently developed methods for systematically altering the myoblast lipid composition, we have obtained evidence suggesting an intimate, direct lipid involvement in myoblast fusion. From these and other observations we have developed a working hypothesis for myoblast fusion in which fusion is a lipid mediated event whose rate is modulated by the physical state of the lipids. The overall objective of this proposal is to test and develop this hypothesis. In the upcoming grant year, we propose to determine the distribution of lipids among the membrane surfaces. We will also explore the changes in membrane fluidity that are alleged to correlate with differentiation and the onset of fusion competence.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM023244-10
Application #
3271563
Study Section
Cognition and Perception Study Section (CP)
Project Start
1976-05-01
Project End
1987-06-30
Budget Start
1985-07-01
Budget End
1986-06-30
Support Year
10
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of Pennsylvania
Department
Type
Schools of Medicine
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104

  Publications

Bachir, Alexia I; Horwitz, Alan Rick; Nelson, W James et al. (2017) Actin-Based Adhesion Modules Mediate Cell Interactions with the Extracellular Matrix and Neighboring Cells. Cold Spring Harb Perspect Biol 9:
Martin-Vilchez, Samuel; Whitmore, Leanna; Asmussen, Hannelore et al. (2017) RhoGTPase Regulators Orchestrate Distinct Stages of Synaptic Development. PLoS One 12:e0170464
Kubow, Kristopher E; Shuklis, Victoria D; Sales, Dominic J et al. (2017) Contact guidance persists under myosin inhibition due to the local alignment of adhesions and individual protrusions. Sci Rep 7:14380
Cross, A M; Wilson, A L; Guerrero, M S et al. (2016) Breast cancer antiestrogen resistance 3-p130(Cas) interactions promote adhesion disassembly and invasion in breast cancer cells. Oncogene 35:5850-5859
Ramos, Grasieli de Oliveira; Bernardi, Lisiane; Lauxen, Isabel et al. (2016) Fibronectin Modulates Cell Adhesion and Signaling to Promote Single Cell Migration of Highly Invasive Oral Squamous Cell Carcinoma. PLoS One 11:e0151338
Horwitz, Rick (2016) Integrated, multi-scale, spatial-temporal cell biology--A next step in the post genomic era. Methods 96:3-5
Devreotes, Peter; Horwitz, Alan Rick (2015) Signaling networks that regulate cell migration. Cold Spring Harb Perspect Biol 7:a005959
Newell-Litwa, Karen A; Badoual, Mathilde; Asmussen, Hannelore et al. (2015) ROCK1 and 2 differentially regulate actomyosin organization to drive cell and synaptic polarity. J Cell Biol 210:225-42
Newell-Litwa, Karen A; Horwitz, Rick; Lamers, Marcelo L (2015) Non-muscle myosin II in disease: mechanisms and therapeutic opportunities. Dis Model Mech 8:1495-515
Juanes-Garcia, Alba; Chapman, Jessica R; Aguilar-Cuenca, Rocio et al. (2015) A regulatory motif in nonmuscle myosin II-B regulates its role in migratory front-back polarity. J Cell Biol 209:23-32

Showing the most recent 10 out of 91 publications