Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
2R01GM023900-19
Application #
2174174
Study Section
Physiological Chemistry Study Section (PC)
Project Start
1977-09-09
Project End
2000-04-30
Budget Start
1996-05-01
Budget End
1997-04-30
Support Year
19
Fiscal Year
1996
Total Cost
Indirect Cost
Name
Wadsworth Center
Department
Type
DUNS #
110521739
City
Menands
State
NY
Country
United States
Zip Code
12204
Trimble, Robert B; Lubowski, Catherine; Hauer 3rd, Charles R et al. (2004) Characterization of N- and O-linked glycosylation of recombinant human bile salt-stimulated lipase secreted by Pichia pastoris. Glycobiology 14:265-74
Andreishcheva, Ekaterina N; Kunkel, Jeremy P; Gemmill, Trent R et al. (2004) Five genes involved in biosynthesis of the pyruvylated Galbeta1,3-epitope in Schizosaccharomyces pombe N-linked glycans. J Biol Chem 279:35644-55
Cipollo, John F; Trimble, Robert B (2002) Hypoglycosylation in the alg12delta yeast mutant destabilizes protease A and causes proteolytic loss of external invertase. Glycobiology 12:30G-3G
Cipollo, John F; Trimble, Robert B (2002) The Saccharomyces cerevisiae alg12delta mutant reveals a role for the middle-arm alpha1,2Man- and upper-arm alpha1,2Manalpha1,6Man- residues of Glc3Man9GlcNAc2-PP-Dol in regulating glycoprotein glycan processing in the endoplasmic reticulum and Golgi appa Glycobiology 12:749-62
Cipollo, J F; Trimble, R B; Chi, J H et al. (2001) The yeast ALG11 gene specifies addition of the terminal alpha 1,2-Man to the Man5GlcNAc2-PP-dolichol N-glycosylation intermediate formed on the cytosolic side of the endoplasmic reticulum. J Biol Chem 276:21828-40
Verostek, M F; Lubowski, C; Trimble, R B (2000) Selective organic precipitation/extraction of released N-glycans following large-scale enzymatic deglycosylation of glycoproteins. Anal Biochem 278:111-22
Cipollo, J F; Trimble, R B (2000) The accumulation of Man(6)GlcNAc(2)-PP-dolichol in the Saccharomyces cerevisiae Deltaalg9 mutant reveals a regulatory role for the Alg3p alpha1,3-Man middle-arm addition in downstream oligosaccharide-lipid and glycoprotein glycan processing. J Biol Chem 275:4267-77
Cipollo, J F; Trimble, R B; Rance, M et al. (2000) Two-dimensional relayed-rotating-frame overhauser spectroscopy (1)H NMR experiments for the selective identification of 1,2-glycosidic linkages in polysaccharides. Anal Biochem 278:52-8
Gemmill, T R; Trimble, R B (1999) Schizosaccharomyces pombe produces novel Gal0-2Man1-3 O-linked oligosaccharides. Glycobiology 9:507-15
Gemmill, T R; Trimble, R B (1999) Overview of N- and O-linked oligosaccharide structures found in various yeast species. Biochim Biophys Acta 1426:227-37

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