Two of the three major objectives of the research program supported by this grant remain unchanged from the original proposal. The first of these involves the use of a combination of biochemical and genetic approaches to decipher the biological role of histone acetylation in higher eukaryotes. The second objective is to uncover some of the molecular details of the process whereby positional information is imparted to cells during embryogenesis, using as a model system the specification of anterio-posterior position by the """"""""zone of polarizing activity"""""""" (ZPA) in the early chick limb bud. A newer objective concerns determining the biological role of mammalian type II DNA topoisomerases, new enzymes recently discovered in this laboratory which we suspect are intimately involved in the initiation of eukaryotic DNA replication.
| Liu, Ling; Su, Xiaoyang; Quinn 3rd, William J et al. (2018) Quantitative Analysis of NAD Synthesis-Breakdown Fluxes. Cell Metab 27:1067-1080.e5 |
| Groen, Aaron C; Mitchison, Timothy J (2016) Purification and Fluorescent Labeling of Tubulin from Xenopus laevis Egg Extracts. Methods Mol Biol 1413:35-45 |
| Özlü, Nurhan; Qureshi, Mohammad H; Toyoda, Yusuke et al. (2015) Quantitative comparison of a human cancer cell surface proteome between interphase and mitosis. EMBO J 34:251-65 |
| Coughlin, Margaret; Groen, Aaron C; Mitchison, Timothy J (2014) Electron microscopy of microtubule cytoskeleton assembly in vitro. Methods Mol Biol 1117:259-71 |
| Mitchison, Timothy J; Nguyen, Phuong; Coughlin, Margaret et al. (2013) Self-organization of stabilized microtubules by both spindle and midzone mechanisms in Xenopus egg cytosol. Mol Biol Cell 24:1559-73 |
| Kueh, Hao Yuan; Niethammer, Philipp; Mitchison, Timothy J (2013) Maintenance of mitochondrial oxygen homeostasis by cosubstrate compensation. Biophys J 104:1338-48 |
| Hu, Chi-Kuo; Ozlu, Nurhan; Coughlin, Margaret et al. (2012) Plk1 negatively regulates PRC1 to prevent premature midzone formation before cytokinesis. Mol Biol Cell 23:2702-11 |
| Hu, Chi-Kuo; Coughlin, Margaret; Mitchison, Timothy J (2012) Midbody assembly and its regulation during cytokinesis. Mol Biol Cell 23:1024-34 |
| Zhang, Xin; Wang, Wenchao; Bedigian, Anne V et al. (2012) Dopamine receptor D3 regulates endocytic sorting by a Prazosin-sensitive interaction with the coatomer COPI. Proc Natl Acad Sci U S A 109:12485-90 |
| Maiuri, Paolo; Terriac, Emmanuel; Paul-Gilloteaux, Perrine et al. (2012) The first World Cell Race. Curr Biol 22:R673-5 |
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