Abstract

The long-term goal of this study is to understand the molecular mechanisms that regulate transcription of the large ribosomal RNAs of the frog, Xenopus laevis. In the next five years efforts will be focused on purification and characterization of trans-acting proteins that interact with already identified DNA sequences to effect this regulation. These studies will include: 1. Purification of accessory proteins that interact with the enhancers and promoters, cloning of their genes, and determination of their mechanism of action. 2. Study of the structure and regulation of RNA polymerase I. 3. Study of the relation between ribosomal RNA transcription and growth control. 4. Study the mechanism of nucleolar assembly-disassembly in vitro. 5. Transform cells to make artificial nucleoli with drug resistant ribosomal genes. 6. Locate origin(s) of replication and study mechanism of rDNA amplification.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM026624-15
Application #
3274038
Study Section
Molecular Cytology Study Section (CTY)
Project Start
1979-08-01
Project End
1994-07-31
Budget Start
1993-08-01
Budget End
1994-07-31
Support Year
15
Fiscal Year
1993
Total Cost
Indirect Cost

  Institution

Name
Fred Hutchinson Cancer Research Center
Department
Type
DUNS #
075524595
City
Seattle
State
WA
Country
United States
Zip Code
98109

  Publications

Aprikian, P; Moorefield, B; Reeder, R H (2001) New model for the yeast RNA polymerase I transcription cycle. Mol Cell Biol 21:4847-55
Aprikian, P; Moorefield, B; Reeder, R H (2000) TATA binding protein can stimulate core-directed transcription by yeast RNA polymerase I. Mol Cell Biol 20:5269-75
Moorefield, B; Greene, E A; Reeder, R H (2000) RNA polymerase I transcription factor Rrn3 is functionally conserved between yeast and human. Proc Natl Acad Sci U S A 97:4724-9
Lin, C W; Moorefield, B; Payne, J et al. (1996) A novel 66-kilodalton protein complexes with Rrn6, Rrn7, and TATA-binding protein to promote polymerase I transcription initiation in Saccharomyces cerevisiae. Mol Cell Biol 16:6436-43
Schultz, M C; Choe, S Y; Reeder, R H (1993) In vitro definition of the yeast RNA polymerase I enhancer. Mol Cell Biol 13:2644-54
Palmer, T D; Miller, A D; Reeder, R H et al. (1993) Efficient expression of a protein coding gene under the control of an RNA polymerase I promoter. Nucleic Acids Res 21:3451-7
Schultz, M C; Brill, S J; Ju, Q et al. (1992) Topoisomerases and yeast rRNA transcription: negative supercoiling stimulates initiation and topoisomerase activity is required for elongation. Genes Dev 6:1332-41
Schultz, M C; Choe, S Y; Reeder, R H (1991) Specific initiation by RNA polymerase I in a whole-cell extract from yeast. Proc Natl Acad Sci U S A 88:1004-8
McStay, B; Hu, C H; Pikaard, C S et al. (1991) xUBF and Rib 1 are both required for formation of a stable polymerase I promoter complex in X. laevis. EMBO J 10:2297-303
McStay, B; Frazier, M W; Reeder, R H (1991) xUBF contains a novel dimerization domain essential for RNA polymerase I transcription. Genes Dev 5:1957-68

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