Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM026762-08
Application #
3274201
Study Section
Physiological Chemistry Study Section (PC)
Project Start
1979-07-01
Project End
1987-08-31
Budget Start
1987-04-01
Budget End
1987-08-31
Support Year
8
Fiscal Year
1987
Total Cost
Indirect Cost
Name
Massachusetts Institute of Technology
Department
Type
Schools of Arts and Sciences
DUNS #
City
Cambridge
State
MA
Country
United States
Zip Code
02139
Yang, Hongying; Roberts, Mary F (2004) Expression and characterization of a heterodimer of Streptomyces chromofuscus phospholipase D. Biochim Biophys Acta 1703:43-51
Zambonelli, Carlo; Casali, Monica; Roberts, Mary F (2003) Mutagenesis of putative catalytic and regulatory residues of Streptomyces chromofuscus phospholipase D differentially modifies phosphatase and phosphodiesterase activities. J Biol Chem 278:52282-9
Yang, Hongying; Roberts, Mary F (2003) Phosphohydrolase and transphosphatidylation reactions of two Streptomyces phospholipase D enzymes: covalent versus noncovalent catalysis. Protein Sci 12:2087-98
Oh, Mi-Kyung; Yang, Hongying; Roberts, Mary F (2003) Using O-(n-alkyl)-N-(N,N'-dimethylethyl)phosphoramidates to investigate the role of Ca2+ and interfacial binding in a bacterial phospholipase D. Biochim Biophys Acta 1649:146-53
Zambonelli, Carlo; Roberts, Mary F (2003) An iron-dependent bacterial phospholipase D reminiscent of purple acid phosphatases. J Biol Chem 278:13706-11
Yang, Hongying; Roberts, Mary F (2002) Cloning, overexpression, and characterization of a bacterial Ca2+-dependent phospholipase D. Protein Sci 11:2958-68
Stieglitz, K A; Seaton, B A; Roberts, M F (2001) Binding of proteolytically processed phospholipase D from Streptomyces chromofuscus to phosphatidylcholine membranes facilitates vesicle aggregation and fusion. Biochemistry 40:13954-63
Geng, D; Baker, D P; Foley, S F et al. (1999) A 20-kDa domain is required for phosphatidic acid-induced allosteric activation of phospholipase D from Streptomyces chromofuscus. Biochim Biophys Acta 1430:234-44
Stieglitz, K; Seaton, B; Roberts, M F (1999) The role of interfacial binding in the activation of Streptomyces chromofuscus phospholipase D by phosphatidic acid. J Biol Chem 274:35367-74
Zhou, C; Horstman, D; Carpenter, G et al. (1999) Action of phosphatidylinositol-specific phospholipase Cgamma1 on soluble and micellar substrates. Separating effects on catalysis from modulation of the surface. J Biol Chem 274:2786-93

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