Abstract

We wish to define the entire domain of the bacteriophage T4 rIIB mRNA that is scanned by the ribosome during the initiation of protein synthesis. We will use exhaustive genetics to select mutations that diminish translation of the rIIB mRNA and biochemistry to determine contacts between ribosomes and the mRNA. We will ask the congruence between the genetic and biochemical answers. Additionally, we will study a translational repressor that interacts with the rIIB mRNA. We will use genetics and biochemistry here as well. Our goal is to understand in molecular terms a set of interactions that control gene expression in prokaryotes at the post-transcriptional level.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM028685-05
Application #
3275946
Study Section
Physiological Chemistry Study Section (PC)
Project Start
1980-12-01
Project End
1985-11-30
Budget Start
1984-12-01
Budget End
1985-11-30
Support Year
5
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of Colorado at Boulder
Department
Type
Schools of Arts and Sciences
DUNS #
City
Boulder
State
CO
Country
United States
Zip Code
80309

  Publications

Morris, K N; Jensen, K B; Julin, C M et al. (1998) High affinity ligands from in vitro selection: complex targets. Proc Natl Acad Sci U S A 95:2902-7
Ringquist, S; Gold, L (1998) Toeprinting assays. Mapping by blocks to reverse transcriptase primer extension. Methods Mol Biol 77:283-95
Chen, H; McBroom, D G; Zhu, Y Q et al. (1996) Inhibitory RNA ligand to reverse transcriptase from feline immunodeficiency virus. Biochemistry 35:6923-30
Jayasena, V K; Brown, D; Shtatland, T et al. (1996) In vitro selection of RNA specifically cleaved by bacteriophage T4 RegB endonuclease. Biochemistry 35:2349-56
He, Y Y; Stockley, P G; Gold, L (1996) In vitro evolution of the DNA binding sites of Escherichia coli methionine repressor, MetJ. J Mol Biol 255:55-66
Chen, H; Brown, D; Gold, L (1996) Novel methods of generating specific oligonucleotide inhibitors of viral polymerases. Methods Enzymol 275:503-20
Nieuwlandt, D; Wecker, M; Gold, L (1995) In vitro selection of RNA ligands to substance P. Biochemistry 34:5651-9
Schneider, D J; Feigon, J; Hostomsky, Z et al. (1995) High-affinity ssDNA inhibitors of the reverse transcriptase of type 1 human immunodeficiency virus. Biochemistry 34:9599-610
Jensen, K B; Atkinson, B L; Willis, M C et al. (1995) Using in vitro selection to direct the covalent attachment of human immunodeficiency virus type 1 Rev protein to high-affinity RNA ligands. Proc Natl Acad Sci U S A 92:12220-4
Binkley, J; Allen, P; Brown, D M et al. (1995) RNA ligands to human nerve growth factor. Nucleic Acids Res 23:3198-205

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