Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
2R01GM028946-16
Application #
2175332
Study Section
Special Emphasis Panel (ZRG5-MBC-1 (01))
Project Start
1981-04-01
Project End
2000-03-31
Budget Start
1996-04-01
Budget End
1997-03-31
Support Year
16
Fiscal Year
1996
Total Cost
Indirect Cost
Name
University of Connecticut
Department
Microbiology/Immun/Virology
Type
Schools of Dentistry
DUNS #
City
Farmington
State
CT
Country
United States
Zip Code
06030
Das, Asis; Garcia Mena, Jaime; Jana, Nandan et al. (2003) Genetic and biochemical strategies to elucidate the architecture and targets of a processive transcription antiterminator from bacteriophage lambda. Methods Enzymol 371:438-59
Kulish, D; Lee, J; Lomakin, I et al. (2000) The functional role of basic patch, a structural element of Escherichia coli transcript cleavage factors GreA and GreB. J Biol Chem 275:12789-98
Toulme, F; Mosrin-Huaman, C; Sparkowski, J et al. (2000) GreA and GreB proteins revive backtracked RNA polymerase in vivo by promoting transcript trimming. EMBO J 19:6853-9
Garcia-Mena, J; Das, A; Sanchez-Trujillo, A et al. (1999) A novel mutation in the KH domain of polynucleotide phosphorylase affects autoregulation and mRNA decay in Escherichia coli. Mol Microbiol 33:235-48
Rees, W A; Weitzel, S E; Das, A et al. (1997) Regulation of the elongation-termination decision at intrinsic terminators by antitermination protein N of phage lambda. J Mol Biol 273:797-813
Van Gilst, M R; Rees, W A; Das, A et al. (1997) Complexes of N antitermination protein of phage lambda with specific and nonspecific RNA target sites on the nascent transcript. Biochemistry 36:1514-24
Das, A; Pal, M; Mena, J G et al. (1996) Components of multiprotein-RNA complex that controls transcription elongation in Escherichia coli phage lambda. Methods Enzymol 274:374-402
Liu, K; Zhang, Y; Severinov, K et al. (1996) Role of Escherichia coli RNA polymerase alpha subunit in modulation of pausing, termination and anti-termination by the transcription elongation factor NusA. EMBO J 15:150-61
Rees, W A; Weitzel, S E; Yager, T D et al. (1996) Bacteriophage lambda N protein alone can induce transcription antitermination in vitro. Proc Natl Acad Sci U S A 93:342-6
Das, A; Barik, S; Ghosh, B et al. (1996) Immunoprinting: a technique used to study dynamic protein-nucleic acid interactions within transcription elongation complex. Methods Enzymol 274:363-74

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