Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM031986-13
Application #
2176391
Study Section
Physical Biochemistry Study Section (PB)
Project Start
1983-04-01
Project End
1998-11-30
Budget Start
1995-12-01
Budget End
1996-11-30
Support Year
13
Fiscal Year
1996
Total Cost
Indirect Cost
Name
State University New York Stony Brook
Department
Biochemistry
Type
Schools of Arts and Sciences
DUNS #
804878247
City
Stony Brook
State
NY
Country
United States
Zip Code
11794
Wang, Jie; London, Erwin (2009) The membrane topography of the diphtheria toxin T domain linked to the a chain reveals a transient transmembrane hairpin and potential translocation mechanisms. Biochemistry 48:10446-56
Lai, Bing; Zhao, Gang; London, Erwin (2008) Behavior of the deeply inserted helices in diphtheria toxin T domain: helices 5, 8, and 9 interact strongly and promote pore formation, while helices 6/7 limit pore formation. Biochemistry 47:4565-74
Fujita, Kentaro; Krishnakumar, Shyam S; Franco, David et al. (2007) Membrane topography of the hydrophobic anchor sequence of poliovirus 3A and 3AB proteins and the functional effect of 3A/3AB membrane association upon RNA replication. Biochemistry 46:5185-99
Buchanan, Susan K; Lukacik, Petra; Grizot, Sylvestre et al. (2007) Structure of colicin I receptor bound to the R-domain of colicin Ia: implications for protein import. EMBO J 26:2594-604
Wu, Zhengyan; Jakes, Karen S; Samelson-Jones, Ben S et al. (2006) Protein translocation by bacterial toxin channels: a comparison of diphtheria toxin and colicin Ia. Biophys J 91:3249-56
Wang, Jie; Rosconi, Michael P; London, Erwin (2006) Topography of the hydrophilic helices of membrane-inserted diphtheria toxin T domain: TH1-TH3 as a hydrophilic tether. Biochemistry 45:8124-34
Zhao, Gang; London, Erwin (2006) An amino acid ""transmembrane tendency"" scale that approaches the theoretical limit to accuracy for prediction of transmembrane helices: relationship to biological hydrophobicity. Protein Sci 15:1987-2001
Musse, Abdiwahab A; Wang, Jie; Deleon, Gladys P et al. (2006) Scanning the membrane-bound conformation of helix 1 in the colicin E1 channel domain by site-directed fluorescence labeling. J Biol Chem 281:885-95
White, Dawn; Musse, Abdiwahab A; Wang, Jie et al. (2006) Toward elucidating the membrane topology of helix two of the colicin E1 channel domain. J Biol Chem 281:32375-84
Zhao, Gang; London, Erwin (2005) Behavior of diphtheria toxin T domain containing substitutions that block normal membrane insertion at Pro345 and Leu307: control of deep membrane insertion and coupling between deep insertion of hydrophobic subdomains. Biochemistry 44:4488-98

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