Abstract

The goal of this research project is identification, isolation, and molecular characterization of the 8-adrenergic receptor. This will be accomplished by the use of specific highly radioactive photoaffinity labelling reagents which are derivations of potent beta-antagonists, such as propranolol and pindolol. Purification of the receptor will be attempted using alprenolol affinity columns. Detergent extracts of membranes containing receptor-beta-receptor antagonist (with biotinyl groups attached) will be passed over the columns to effect purification of the receptor. Other aspects of this proposal involve investigations on the involvement of sulfhydryl groups in the mechanism by which catecholamines activate adenylate cyclase. Distribution of the receptor on the surface of cells will be investigated using bifunctional ligands containing biotin and the protein, avidin, suitably derivated for microscope visualization.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM033138-12
Application #
3282477
Study Section
Pharmacology A Study Section (PHRA)
Project Start
1983-07-01
Project End
1988-06-30
Budget Start
1986-07-01
Budget End
1987-06-30
Support Year
12
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
University of Wisconsin Madison
Department
Type
Schools of Medicine
DUNS #
161202122
City
Madison
State
WI
Country
United States
Zip Code
53715

  Publications

Chu, Uyen B; Ramachandran, Subramaniam; Hajipour, Abdol R et al. (2013) Photoaffinity labeling of the sigma-1 receptor with N-[3-(4-nitrophenyl)propyl]-N-dodecylamine: evidence of receptor dimers. Biochemistry 52:859-68
Ruoho, Arnold E; Chu, Uyen B; Ramachandran, Subramaniam et al. (2012) The ligand binding region of the sigma-1 receptor: studies utilizing photoaffinity probes, sphingosine and N-alkylamines. Curr Pharm Des 18:920-9
Chu, Uyen B; Hajipour, Abdol R; Ramachandran, Subramaniam et al. (2011) Characterization of interactions of 4-nitrophenylpropyl-N-alkylamine with ýý receptors. Biochemistry 50:7568-78
Guo, Lian-Wang; Ruoho, Arnold E (2011) N-terminal half of the cGMP phosphodiesterase gamma-subunit contributes to stabilization of the GTPase-accelerating protein complex. J Biol Chem 286:15260-7
Mavlyutov, Timur A; Nickells, Robert W; Guo, Lian-Wang (2011) Accelerated retinal ganglion cell death in mice deficient in the Sigma-1 receptor. Mol Vis 17:1034-43
Zahmatkesh, Saeed; Hajipour, Abdol R (2010) Microwave-assisted synthesis and characterization of optically active poly (ester-imide)s incorporating L-alanine. Amino Acids 38:1253-60
Chu, Uyen B; Song, Jikui; Mavlyutov, Timur A et al. (2010) In vitro interaction of tubulin with the photoreceptor cGMP phosphodiesterase gamma-subunit. Neurosci Lett 482:225-9
Guo, Lian-Wang; Hajipour, Abdol R; Ruoho, Arnold E (2010) Complementary interactions of the rod PDE6 inhibitory subunit with the catalytic subunits and transducin. J Biol Chem 285:15209-19
Hajipour, Abdol R; Zahmatkesh, Saeed; Roosta, Parniyan et al. (2009) Synthesis and characterization of new optically active poly(azo-ester-imide)s via interfacial polycondensation. Amino Acids 36:511-8
Guo, Lian-Wang; Ruoho, Arnold E (2008) The retinal cGMP phosphodiesterase gamma-subunit - a chameleon. Curr Protein Pept Sci 9:611-25

Showing the most recent 10 out of 47 publications