Abstract

The long-term goal of this project is to understand the molecular mechanisms which cause embryonic cells to adopt particular fates as a function of their position in the embryo. The process to be studied is the establishment of the dorsal-ventral pattern of the Drosophila embryo, where genetic, embryological and molecular techniques can be combined to characterize the function of individual gene products. One maternal effect gene which appears to play a central role in the establishment of embryonic dorsal-ventral polarity, Toll, will be studied in detail. Genetic studies will include isolating new recessive Toll alleles and studying the phenotypes produced by Toll alleles combined with other mutations affecting the embryonic dorsal-ventral pattern. Mutant alleles of Toll will be characterized by their phenotype and by the response of mutant embryos to the injection of wild type cytoplasm. The Toll gene will be cloned, and mutations which perturb Toll function in defined ways will be mapped onto the DNA.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM035437-02
Application #
3288189
Study Section
Genetics Study Section (GEN)
Project Start
1985-07-01
Project End
1988-06-30
Budget Start
1986-07-01
Budget End
1987-06-30
Support Year
2
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
University of California Berkeley
Department
Type
Schools of Arts and Sciences
DUNS #
094878337
City
Berkeley
State
CA
Country
United States
Zip Code
94704

  Publications

Misra, S; Hecht, P; Maeda, R et al. (1998) Positive and negative regulation of Easter, a member of the serine protease family that controls dorsal-ventral patterning in the Drosophila embryo. Development 125:1261-7
Wu, L P; Anderson, K V (1997) Related signaling networks in Drosophila that control dorsoventral patterning in the embryo and the immune response. Cold Spring Harb Symp Quant Biol 62:97-103
Schneider, D S; Jin, Y; Morisato, D et al. (1994) A processed form of the Spatzle protein defines dorsal-ventral polarity in the Drosophila embryo. Development 120:1243-50
Morisato, D; Anderson, K V (1994) The spatzle gene encodes a component of the extracellular signaling pathway establishing the dorsal-ventral pattern of the Drosophila embryo. Cell 76:677-88
Ferguson, E L; Anderson, K V (1992) Decapentaplegic acts as a morphogen to organize dorsal-ventral pattern in the Drosophila embryo. Cell 71:451-61
Ferguson, E L; Anderson, K V (1992) Localized enhancement and repression of the activity of the TGF-beta family member, decapentaplegic, is necessary for dorsal-ventral pattern formation in the Drosophila embryo. Development 114:583-97
Chasan, R; Jin, Y; Anderson, K V (1992) Activation of the easter zymogen is regulated by five other genes to define dorsal-ventral polarity in the Drosophila embryo. Development 115:607-16
Hashimoto, C; Gerttula, S; Anderson, K V (1991) Plasma membrane localization of the Toll protein in the syncytial Drosophila embryo: importance of transmembrane signaling for dorsal-ventral pattern formation. Development 111:1021-8
Shimell, M J; Ferguson, E L; Childs, S R et al. (1991) The Drosophila dorsal-ventral patterning gene tolloid is related to human bone morphogenetic protein 1. Cell 67:469-81
Schneider, D S; Hudson, K L; Lin, T Y et al. (1991) Dominant and recessive mutations define functional domains of Toll, a transmembrane protein required for dorsal-ventral polarity in the Drosophila embryo. Genes Dev 5:797-807

Showing the most recent 10 out of 14 publications