Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM035654-11
Application #
2177990
Study Section
Microbial Physiology and Genetics Subcommittee 2 (MBC)
Project Start
1990-01-01
Project End
1997-12-31
Budget Start
1996-01-01
Budget End
1996-12-31
Support Year
11
Fiscal Year
1996
Total Cost
Indirect Cost
Name
University of Texas Austin
Department
Microbiology/Immun/Virology
Type
Schools of Arts and Sciences
DUNS #
City
Austin
State
TX
Country
United States
Zip Code
78712
Rowley, Paul A; Kachroo, Aashiq H; Ma, Chien-Hui et al. (2010) Electrostatic suppression allows tyrosine site-specific recombination in the absence of a conserved catalytic arginine. J Biol Chem 285:22976-85
Kachroo, Aashiq H; Ma, Chien-Hui; Rowley, Paul A et al. (2010) Restoration of catalytic functions in Cre recombinase mutants by electrostatic compensation between active site and DNA substrate. Nucleic Acids Res 38:6589-601
Ma, Chien-Hui; Rowley, Paul A; Macieszak, Anna et al. (2009) Active site electrostatics protect genome integrity by blocking abortive hydrolysis during DNA recombination. EMBO J 28:1745-56
Ma, Chien-Hui; Kachroo, Aashiq H; Macieszak, Anna et al. (2009) Reactions of Cre with methylphosphonate DNA: similarities and contrasts with Flp and vaccinia topoisomerase. PLoS One 4:e7248
Ma, Chien-Hui; Kwiatek, Agnieszka; Bolusani, Swetha et al. (2007) Unveiling hidden catalytic contributions of the conserved His/Trp-III in tyrosine recombinases: assembly of a novel active site in Flp recombinase harboring alanine at this position. J Mol Biol 368:183-96
Du, Quan; Livshits, Alexei; Kwiatek, Agnieszka et al. (2007) Protein-induced local DNA bends regulate global topology of recombination products. J Mol Biol 368:170-82
Harshey, Rasika M; Jayaram, Makkuni (2006) The mu transpososome through a topological lens. Crit Rev Biochem Mol Biol 41:387-405
Bolusani, Swetha; Ma, Chien-Hui; Paek, Andrew et al. (2006) Evolution of variants of yeast site-specific recombinase Flp that utilize native genomic sequences as recombination target sites. Nucleic Acids Res 34:5259-69
Yin, Zhiqi; Jayaram, Makkuni; Pathania, Shailja et al. (2005) The Mu transposase interwraps distant DNA sites within a functional transpososome in the absence of DNA supercoiling. J Biol Chem 280:6149-56
Konieczka, Jay H; Paek, Andrew; Jayaram, Makkuni et al. (2004) Recombination of hybrid target sites by binary combinations of Flp variants: mutations that foster interprotomer collaboration and enlarge substrate tolerance. J Mol Biol 339:365-78

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