Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM036065-10
Application #
2178206
Study Section
Neurology C Study Section (NEUC)
Project Start
1981-06-01
Project End
1997-05-31
Budget Start
1995-06-01
Budget End
1997-05-31
Support Year
10
Fiscal Year
1995
Total Cost
Indirect Cost
Name
University of Kentucky
Department
Biochemistry
Type
Schools of Medicine
DUNS #
832127323
City
Lexington
State
KY
Country
United States
Zip Code
40506
Rush, Jeffrey S (2015) Role of Flippases in Protein Glycosylation in the Endoplasmic Reticulum. Lipid Insights 8:45-53
Harrison, Kenneth D; Park, Eon Joo; Gao, Ningguo et al. (2011) Nogo-B receptor is necessary for cellular dolichol biosynthesis and protein N-glycosylation. EMBO J 30:2490-500
Rush, Jeffrey S; Alaimo, Cristina; Robbiani, Riccardo et al. (2010) A novel epimerase that converts GlcNAc-P-P-undecaprenol to GalNAc-P-P-undecaprenol in Escherichia coli O157. J Biol Chem 285:1671-80
Rush, Jeffrey S; Gao, Ningguo; Lehrman, Mark A et al. (2009) Suppression of Rft1 expression does not impair the transbilayer movement of Man5GlcNAc2-P-P-dolichol in sealed microsomes from yeast. J Biol Chem 284:19835-42
Hartman, Matthew C T; Jiang, Songmin; Rush, Jeffrey S et al. (2007) Glycosyltransferase mechanisms: impact of a 5-fluoro substituent in acceptor and donor substrates on catalysis. Biochemistry 46:11630-8
Rush, Jeffrey S; Waechter, Charles J (2006) Partial purification of mannosylphosphorylundecaprenol synthase from Micrococcus luteus: a useful enzyme for the biosynthesis of a variety of mannosylphosphorylpolyisoprenol products. Methods Mol Biol 347:13-30
Pakkiri, Leroy S; Waechter, Charles J (2005) Dimannosyldiacylglycerol serves as a lipid anchor precursor in the assembly of the membrane-associated lipomannan in Micrococcus luteus. Glycobiology 15:291-302
Rush, Jeffrey S; Waechter, C J (2005) Assay for the transbilayer movement of polyisoprenoid-linked saccharides based on the transport of water-soluble analogues. Methods 35:316-22
Rush, Jeffrey S; Waechter, C J (2004) Functional reconstitution into proteoliposomes and partial purification of a rat liver ER transport system for a water-soluble analogue of mannosylphosphoryldolichol. Biochemistry 43:7643-52
Fernandez, F; Rush, J S; Toke, D A et al. (2001) The CWH8 gene encodes a dolichyl pyrophosphate phosphatase with a luminally oriented active site in the endoplasmic reticulum of Saccharomyces cerevisiae. J Biol Chem 276:41455-64

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