Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM036325-12
Application #
2178301
Study Section
Metallobiochemistry Study Section (BMT)
Project Start
1985-08-01
Project End
1999-07-31
Budget Start
1996-08-01
Budget End
1997-07-31
Support Year
12
Fiscal Year
1996
Total Cost
Indirect Cost
Name
Scripps Research Institute
Department
Type
DUNS #
City
La Jolla
State
CA
Country
United States
Zip Code
92037
Jung, Y S; Bonagura, C A; Tilley, G J et al. (2000) Structure of C42D Azotobacter vinelandii FdI. A Cys-X-X-Asp-X-X-Cys motif ligates an air-stable [4Fe-4S]2+/+ cluster. J Biol Chem 275:36974-83
Lloyd, S J; Lauble, H; Prasad, G S et al. (1999) The mechanism of aconitase: 1.8 A resolution crystal structure of the S642a:citrate complex. Protein Sci 8:2655-62
Schipke, C G; Goodin, D B; McRee, D E et al. (1999) Oxidized and reduced Azotobacter vinelandii ferredoxin I at 1.4 A resolution: conformational change of surface residues without significant change in the [3Fe-4S]+/0 cluster. Biochemistry 38:8228-39
Chen, K; Tilley, G J; Sridhar, V et al. (1999) Alteration of the reduction potential of the [4Fe-4S](2+/+) cluster of Azotobacter vinelandii ferredoxin I. J Biol Chem 274:36479-87
Gao-Sheridan, H S; Kemper, M A; Khayat, R et al. (1998) A T14C variant of Azotobacter vinelandii ferredoxin I undergoes facile [3Fe-4S]0 to [4Fe-4S]2+ conversion in vitro but not in vivo. J Biol Chem 273:33692-701
Sridhar Prasad, G; Kresge, N; Muhlberg, A B et al. (1998) The crystal structure of NADPH:ferredoxin reductase from Azotobacter vinelandii. Protein Sci 7:2541-9
Stout, C D; Stura, E A; McRee, D E (1998) Structure of Azotobacter vinelandii 7Fe ferredoxin at 1.35 A resolution and determination of the [Fe-S] bonds with 0.01 A accuracy. J Mol Biol 278:629-39
Kemper, M A; Stout, C D; Lloyd, S J et al. (1997) Y13C Azotobacter vinelandii ferredoxin I. A designed [Fe-S] ligand motif contains a cysteine persulfide. J Biol Chem 272:15620-7
Lauble, H; Kennedy, M C; Emptage, M H et al. (1996) The reaction of fluorocitrate with aconitase and the crystal structure of the enzyme-inhibitor complex. Proc Natl Acad Sci U S A 93:13699-703
Lauble, H; Stout, C D (1995) Steric and conformational features of the aconitase mechanism. Proteins 22:1-11

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