Abstract

The proposed research is a biochemical-genetic study of mitochondrial rRNA splicing in Neurospora. The gene encoding the mitochondrial large (25 S) rRNA of Neurospora contains an intervening sequence of ca. 2.3 kb. In previous work, we identified two allelic, nuclear mutants (299-9 and 286-67) which have a temperature-sensitive defect in splicing this RNA. When grown at the nonpermissive temperature (37 degrees), the mutants accumulate a 35 S pre-rRNA which is a colinear transcript of the 25 s RNA gene including the intervening sequence. We have now identified a new nuclear mutant (AEG-193) which also accumulates 35 S RNA, but which is not allelic with the previously studied mutants.
Specific aims of the proposed research are: (a) to continue to characterize the mutants, (b) to continue to isolate and characterize revertants of mutants, (c) to characterize the tertiary structure of 35 S RNA and 35 S RNA-ribonucleoprotein particles, and (d) to develop techniques for assay purification of the RNA splicing enzymes. These studies are intended to provide insight into the mechanism and physiological role of RNA splicing which should be relevant to all eukaryotic cells.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
7R01GM037951-01
Application #
3293857
Study Section
Genetics Study Section (GEN)
Project Start
1986-09-01
Project End
1988-03-31
Budget Start
1986-09-01
Budget End
1987-03-31
Support Year
1
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
Ohio State University
Department
Type
Schools of Arts and Sciences
DUNS #
098987217
City
Columbus
State
OH
Country
United States
Zip Code
43210

  Publications

Mohr, Georg; Kang, Sean Yoon-Seo; Park, Seung Kuk et al. (2018) A Highly Proliferative Group IIC Intron from Geobacillus stearothermophilus Reveals New Features of Group II Intron Mobility and Splicing. J Mol Biol 430:2760-2783
Stamos, Jennifer L; Lentzsch, Alfred M; Lambowitz, Alan M (2017) Structure of a Thermostable Group II Intron Reverse Transcriptase with Template-Primer and Its Functional and Evolutionary Implications. Mol Cell 68:926-939.e4
Zubradt, Meghan; Gupta, Paromita; Persad, Sitara et al. (2017) DMS-MaPseq for genome-wide or targeted RNA structure probing in vivo. Nat Methods 14:75-82
Bazzini, Ariel A; Del Viso, Florencia; Moreno-Mateos, Miguel A et al. (2016) Codon identity regulates mRNA stability and translation efficiency during the maternal-to-zygotic transition. EMBO J 35:2087-2103
Burke, James M; Kincaid, Rodney P; Nottingham, Ryan M et al. (2016) DUSP11 activity on triphosphorylated transcripts promotes Argonaute association with noncanonical viral microRNAs and regulates steady-state levels of cellular noncoding RNAs. Genes Dev 30:2076-2092
Nottingham, Ryan M; Wu, Douglas C; Qin, Yidan et al. (2016) RNA-seq of human reference RNA samples using a thermostable group II intron reverse transcriptase. RNA 22:597-613
Silas, Sukrit; Mohr, Georg; Sidote, David J et al. (2016) Direct CRISPR spacer acquisition from RNA by a natural reverse transcriptase-Cas1 fusion protein. Science 351:aad4234
Qin, Yidan; Yao, Jun; Wu, Douglas C et al. (2016) High-throughput sequencing of human plasma RNA by using thermostable group II intron reverse transcriptases. RNA 22:111-28
Lamech, Lilian T; Saoji, Maithili; Paukstelis, Paul J et al. (2016) Structural Divergence of the Group I Intron Binding Surface in Fungal Mitochondrial Tyrosyl-tRNA Synthetases That Function in RNA Splicing. J Biol Chem 291:11911-27
Lambowitz, Alan M; Belfort, Marlene (2015) Mobile Bacterial Group II Introns at the Crux of Eukaryotic Evolution. Microbiol Spectr 3:

Showing the most recent 10 out of 54 publications