Several regulators of meiosis in S cerevisiae act on the Rim101 pathway, a conserved signal transduction pathway. Homologous pathways promote expression of genes in response to external alkaline pH in other fungi and, as these investigators have recently found, in yeast as well. External pH is a biological signal that governs growth, physiology, and differentiation of virtually all cells, yet there is limited information about the transcriptional responses to external pH changes. The long-term objective of this study is to determine how external pH changes govern responses at the level of gene expression and cellular differentiation. The immediate goal is to define the mechanisms of signal transduction through and output of the Rim101 pathway, and to use this information to develop a framework for understanding the pH response and its relationship to meiosis.
Specific aims are to determine the functional relationships among Rim101 pathway members, to determine how the Rim101 pathway governs target gene expression, and to determine the relationship between the Rim101 pathway and functional suppressor genes that can promote alkaline pH responses and meiosis.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
2R01GM039531-14
Application #
6266871
Study Section
Microbial Physiology and Genetics Subcommittee 2 (MBC)
Program Officer
Anderson, James J
Project Start
1988-02-03
Project End
2005-01-31
Budget Start
2001-02-01
Budget End
2002-01-31
Support Year
14
Fiscal Year
2001
Total Cost
$385,500
Indirect Cost
Name
Columbia University (N.Y.)
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
167204994
City
New York
State
NY
Country
United States
Zip Code
10032
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Blumental-Perry, Anna; Li, Weishi; Simchen, Giora et al. (2002) Repression and activation domains of RME1p structurally overlap, but differ in genetic requirements. Mol Biol Cell 13:1709-21

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