No pharmacologic agent is known to accelerate and improve the quality of human wound healing in vivo. In preliminary work recombinant EGF accelerated the quality of experimental and human wound healing. Now that milligram quantities of EGF are available it is possible to determine mechanisms whereby EGF and related growth factors affect wound healing in vivo. This application will study processes mediating growth and proliferation of mammalian skin as they relate to wound healing in an in vivo porcine model and where possible in human patients.
The specific aims are: 1) To extend histochemical, EM and morphometric studies using EGF and its receptor to define involvement of this factor in porcine and human wound healing; 2) To determine the sequential appearance and identity of cells activated during wound healing by EGF and other growth factors using binding, phosphorylation, and in situ autoradiography; 3) To determine by in situ hybridization if EGF-R during wound healing is modulated by receptor occupancy, ligand (EGF, TGF-A) or interactions with other factors (TGF-B); 4) To study the effects of EGF and related factors on epithelial and mesenchymal interactions by studying the sequential appearance, distribution and nature of extracellular matrix components namely collagens and fibronectin.
| Greco 3rd, J A; Pollins, A C; Boone, B E et al. (2010) A microarray analysis of temporal gene expression profiles in thermally injured human skin. Burns 36:192-204 |
| Caldwell, Robert L; Opalenik, Susan R; Davidson, Jeffrey M et al. (2008) Tissue profiling MALDI mass spectrometry reveals prominent calcium-binding proteins in the proteome of regenerative MRL mouse wounds. Wound Repair Regen 16:442-9 |
| Nanney, Lillian B; Woodrell, Christopher D; Greives, Mathew R et al. (2008) Calreticulin enhances porcine wound repair by diverse biological effects. Am J Pathol 173:610-30 |
| Pollins, Alonda C; Friedman, David B; Nanney, Lillian B (2007) Proteomic investigation of human burn wounds by 2D-difference gel electrophoresis and mass spectrometry. J Surg Res 142:143-52 |
| Caldwell, Robert L; Opalenik, Susan R; Davidson, Jeffrey M et al. (2007) Tissue profiling MALDI mass spectrometry reveals prominent calcium-binding proteins in the proteome of regenerative MRL mouse wounds. Wound Repair Regen : |
| Okwueze, Martina I; Cardwell, Nancy L; Pollins, Alonda C et al. (2007) Modulation of porcine wound repair with a transfected ErbB3 gene and relevant EGF-like ligands. J Invest Dermatol 127:1030-41 |
| Ajmal, Nadeem; Riordan, Colin L; Cardwell, Nancy et al. (2003) Chemically assisted capsulectomy in the rabbit model: a new approach. Plast Reconstr Surg 112:1449-54; discussion 1462-3 |
| Draper, Bradley K; Komurasaki, Toshi; Davidson, Mari K et al. (2003) Topical epiregulin enhances repair of murine excisional wounds. Wound Repair Regen 11:188-97 |
| Ajmal, Nadeem; Riordan, Colin L; Cardwell, Nancy et al. (2003) The effectiveness of sodium 2-mercaptoethane sulfonate (mesna) in reducing capsular formation around implants in a rabbit model. Plast Reconstr Surg 112:1455-61; discussion 1462-3 |
| Milatovic, Snjezana; Nanney, Lillian B; Yu, Yingchun et al. (2003) Impaired healing of nitrogen mustard wounds in CXCR2 null mice. Wound Repair Regen 11:213-9 |
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