Abstract

The REC1 and REC2 genes of the yeast-like fungus Ustilago maydis exert far- reaching control over DNA repair and recombination. The genes have been cloned, the DNA sequences have been determined, and the deduced amino acid sequences of the encoded proteins have been analyzed. The REC1 gene product is predicted to be a 3'-5' exonuclease. The REC2 gene product is predicted to be a recA-like homologous pairing protein. The gene products have been purified and overexpressed and direct biochemical analysis has confirmed the predicted gene functions. Proposed studies include further experimentation to explore the function of the proteins and to dissect the transcription units of the genes.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM042482-06
Application #
2181413
Study Section
Molecular Biology Study Section (MBY)
Project Start
1989-07-01
Project End
1996-06-30
Budget Start
1994-07-01
Budget End
1995-06-30
Support Year
6
Fiscal Year
1994
Total Cost
Indirect Cost

  Institution

Name
Weill Medical College of Cornell University
Department
Microbiology/Immun/Virology
Type
Schools of Medicine
DUNS #
201373169
City
New York
State
NY
Country
United States
Zip Code
10065

  Publications

de Sena-Tomás, Carmen; Yu, Eun Young; Calzada, Arturo et al. (2015) Fungal Ku prevents permanent cell cycle arrest by suppressing DNA damage signaling at telomeres. Nucleic Acids Res 43:2138-51
de Sena-Tomás, Carmen; Sutherland, Jeanette H; Milisavljevic, Mira et al. (2015) LAMMER kinase contributes to genome stability in Ustilago maydis. DNA Repair (Amst) 33:70-7
Zhou, Qingwen; Holloman, William K (2014) Dual DNA-binding domains shape the interaction of Brh2 with DNA. DNA Repair (Amst) 22:104-11
Kojic, Milorad; Sutherland, Jeanette H; Pérez-Martín, José et al. (2013) Initiation of meiotic recombination in Ustilago maydis. Genetics 195:1231-40
Yu, Eun Young; Kojic, Milorad; Holloman, William K et al. (2013) Brh2 and Rad51 promote telomere maintenance in Ustilago maydis, a new model system of DNA repair proteins at telomeres. DNA Repair (Amst) 12:472-9
Kojic, Milorad; Holloman, William K (2012) Brh2 domain function distinguished by differential cellular responses to DNA damage and replication stress. Mol Microbiol 83:351-61
Zhou, Qingwen; Kojic, Milorad; Holloman, William K (2012) Dss1 release activates DNA binding potential in Brh2. Biochemistry 51:9137-46
Holloman, William K (2011) Unraveling the mechanism of BRCA2 in homologous recombination. Nat Struct Mol Biol 18:748-54
Kojic, Milorad; Zhou, Qingwen; Fan, Jie et al. (2011) Mutational analysis of Brh2 reveals requirements for compensating mediator functions. Mol Microbiol 79:180-91
de Sena-Tomás, Carmen; Fernández-Álvarez, Alfonso; Holloman, William K et al. (2011) The DNA damage response signaling cascade regulates proliferation of the phytopathogenic fungus Ustilago maydis in planta. Plant Cell 23:1654-65

Showing the most recent 10 out of 41 publications