Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM043479-07
Application #
2182025
Study Section
Physical Biochemistry Study Section (PB)
Project Start
1990-07-01
Project End
1999-06-30
Budget Start
1996-07-01
Budget End
1997-06-30
Support Year
7
Fiscal Year
1996
Total Cost
Indirect Cost
Name
University of Texas Sw Medical Center Dallas
Department
Biochemistry
Type
Schools of Medicine
DUNS #
City
Dallas
State
TX
Country
United States
Zip Code
75390
Davydov, Dmitri R; Ponomarev, Gelii V; Bobrovnikova-Marjon, Ekaterina et al. (2013) Aluminum-substituted heme domain of P450BM-3 (BMP): introducing a heme-derived fluorescent probe for studies of substrate binding and protein-protein interactions in cytochromes P450. Biotechnol Appl Biochem 60:41-51
Haines, Donovan C; Hegde, Amita; Chen, Baozhi et al. (2011) A single active-site mutation of P450BM-3 dramatically enhances substrate binding and rate of product formation. Biochemistry 50:8333-41
Haines, Donovan C; Chen, Baozhi; Tomchick, Diana R et al. (2008) Crystal structure of inhibitor-bound P450BM-3 reveals open conformation of substrate access channel. Biochemistry 47:3662-70
Kitazume, Tatsuya; Haines, Donovan C; Estabrook, Ronald W et al. (2007) Obligatory intermolecular electron-transfer from FAD to FMN in dimeric P450BM-3. Biochemistry 46:11892-901
Hegde, Amita; Haines, Donovan C; Bondlela, Muralidhar et al. (2007) Interactions of substrates at the surface of P450s can greatly enhance substrate potency. Biochemistry 46:14010-7
Chowdhary, Puneet K; Keshavan, Neela; Nguyen, Hien Q et al. (2007) Bacillus megaterium CYP102A1 oxidation of acyl homoserine lactones and acyl homoserines. Biochemistry 46:14429-37
Mast, Natalia; Murtazina, Dilyara; Liu, Hong et al. (2006) Distinct binding of cholesterol and 5beta-cholestane-3alpha,7alpha,12alpha-triol to cytochrome P450 27A1: evidence from modeling and site-directed mutagenesis studies. Biochemistry 45:4396-404
Mast, Natalia; Graham, Sandra E; Andersson, Ulla et al. (2005) Cholesterol binding to cytochrome P450 7A1, a key enzyme in bile acid biosynthesis. Biochemistry 44:3259-71
Conley, Alan; Mapes, Samantha; Corbin, C Jo et al. (2002) Structural determinants of aromatase cytochrome p450 inhibition in substrate recognition site-1. Mol Endocrinol 16:1456-68
Graham, Sandra E; Peterson, Julian A (2002) Sequence alignments, variabilities, and vagaries. Methods Enzymol 357:15-28

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