This is a continuation application, centering on the role of HIV capsid protein (CA) in virion structure and post-assembly events, and how the host protein cyclophilin A (CyP A) facilitates this role.
The specific aims of the projects are:
AIM 1 : to identify the basis of Gag (precursor) and CA recognition by cellular chaperons such as CyP A and to determine the role of CyP A in maturation of the Gag precursor.
AIM 2 : to identify the CyP A-dependent CA proteins in viral particles and to determine their function, through reconstitution experiments.
AIM 3 : to define, using X-ray structure and genetic analysis, the structural changes that result from CyP A-induced alterations of the mature CA protein.
AIM 4 : to distinguish regions in the Gag and CA proteins involved in different CyP A interactions, using mutants defective in folding and assays distinguishing the chaperon versus isomerization activities of CyP A.
| Watanabe, Susan M; Medina, Gisselle N; Eastep, Gunnar N et al. (2018) The matrix domain of the Gag protein from avian sarcoma virus contains a PI(4,5)P2-binding site that targets Gag to the cell periphery. J Biol Chem 293:18841-18853 |
| Medina, Gisselle; Zhang, Yongjun; Tang, Yi et al. (2005) The functionally exchangeable L domains in RSV and HIV-1 Gag direct particle release through pathways linked by Tsg101. Traffic 6:880-94 |
